PMID- 20861248
OWN - NLM
STAT- MEDLINE
DCOM- 20101220
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 84
IP  - 23
DP  - 2010 Dec
TI  - Immunization with different viral antigens alters the pattern of T cell 
      exhaustion and latency in herpes simplex virus type 1-infected mice.
PG  - 12315-24
LID - 10.1128/JVI.01600-10 [doi]
AB  - We have shown previously that immunization with herpes simplex virus type 1 
      (HSV-1) glycoprotein K (gK) exacerbated corneal scarring (CS) in ocularly 
      infected mice. In this study, we investigated whether higher levels of CS were 
      correlated with higher levels of latency and T cell exhaustion in gK-immunized 
      mice. BALB/c mice were vaccinated with baculovirus-expressed gK or gD or mock 
      immunized. Twenty-one days after the third immunization, mice were ocularly 
      infected with 2 x 10(4) PFU/eye of virulent HSV-1 strain McKrae. On day 5 
      postinfection, virus replication in the eye was measured, and on day 30 
      postinfection, infiltration of the trigeminal ganglia (TG) by CD4, CD8, 
      programmed death 1 (PD-1), and T cell immunoglobulin and mucin domain-containing 
      protein 3 (Tim-3) was monitored by immunohistochemistry and quantitative 
      real-time PCR (qRT-PCR). This study demonstrated that higher levels of CS were 
      correlated with higher levels of latency, and this was associated with the 
      presence of significantly higher numbers of CD4(+)PD-1(+) and CD8(+)PD-1(+) cells 
      in the TG of the gK-immunized group than in both the gD- and mock-immunized 
      groups. Levels of exhaustion associated with Tim-3 were the same among gK- and 
      mock-vaccinated groups but higher than levels in the gD-vaccinated group. In this 
      study, we have shown for the first time that both PD-1 and Tim-3 contribute to T 
      cell exhaustion and an increase of latency in the TG of latently infected mice.
FAU - Allen, Sariah J
AU  - Allen SJ
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratories, Department of Surgery, CSMC Burns & Allen Research Institute, Los 
      Angeles, California 90048, USA.
FAU - Mott, Kevin R
AU  - Mott KR
FAU - Zandian, Mandana
AU  - Zandian M
FAU - Ghiasi, Homayon
AU  - Ghiasi H
LA  - eng
GR  - R01 EY013615/EY/NEI NIH HHS/United States
GR  - EY13615/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100922
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Antigens, Viral)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Havcr2 protein, mouse)
RN  - 0 (Hepatitis A Virus Cellular Receptor 2)
RN  - 0 (Pdcd1 protein, mouse)
RN  - 0 (Programmed Cell Death 1 Receptor)
RN  - 0 (Receptors, Virus)
RN  - 0 (UL53 protein, Human herpesvirus 1)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Antigens, Differentiation/metabolism
MH  - Antigens, Viral/administration & dosage/*immunology
MH  - CD4 Antigens/metabolism
MH  - CD8 Antigens/metabolism
MH  - Cicatrix/pathology/*virology
MH  - Cornea/pathology/*virology
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Hepatitis A Virus Cellular Receptor 2
MH  - Herpes Simplex/*immunology/prevention & control
MH  - Herpesvirus 1, Human/genetics/*immunology/physiology
MH  - Immunization/*adverse effects
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polymerase Chain Reaction
MH  - Programmed Cell Death 1 Receptor
MH  - Receptors, Virus/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trigeminal Ganglion/metabolism
MH  - Viral Proteins/administration & dosage/*immunology
MH  - Virus Latency/*immunology
MH  - Virus Replication/physiology
PMC - PMC2976415
EDAT- 2010/09/24 06:00
MHDA- 2010/12/21 06:00
PMCR- 2011/06/01
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2010/12/21 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - JVI.01600-10 [pii]
AID - 1600-10 [pii]
AID - 10.1128/JVI.01600-10 [doi]
PST - ppublish
SO  - J Virol. 2010 Dec;84(23):12315-24. doi: 10.1128/JVI.01600-10. Epub 2010 Sep 22.