PMID- 20861331
OWN - NLM
STAT- MEDLINE
DCOM- 20110209
LR  - 20211020
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 17
IP  - 11
DP  - 2010 Nov
TI  - Enzyme-linked immunosorbent assay for detection of filovirus species-specific 
      antibodies.
PG  - 1723-8
LID - 10.1128/CVI.00170-10 [doi]
AB  - Several enzyme-linked immunosorbent assays (ELISAs) for the detection of 
      filovirus-specific antibodies have been developed. However, diagnostic methods to 
      distinguish antibodies specific to the respective species of filoviruses, which 
      provide the basis for serological classification, are not readily available. We 
      established an ELISA using His-tagged secreted forms of the transmembrane 
      glycoproteins (GPs) of five different Ebola virus (EBOV) species and one Marburg 
      virus (MARV) strain as antigens for the detection of filovirus species-specific 
      antibodies. The GP-based ELISA was evaluated by testing antisera collected from 
      mice immunized with virus-like particles as well as from humans and nonhuman 
      primates infected with EBOV or MARV. In our ELISA, little cross-reactivity of IgG 
      antibodies was observed in most of the mouse antisera. Although sera and plasma 
      from some patients and monkeys showed notable cross-reactivity with the GPs from 
      multiple filovirus species, the highest reactions of IgG were uniformly detected 
      against the GP antigen homologous to the virus species that infected individuals. 
      We further confirmed that MARV-specific IgM antibodies were specifically detected 
      in specimens collected from patients during the acute phase of infection. These 
      results demonstrate the usefulness of our ELISA for diagnostics as well as 
      ecological and serosurvey studies.
FAU - Nakayama, Eri
AU  - Nakayama E
AD  - Department of Global Epidemiology, Hokkaido University Research Center for 
      Zoonosis Control, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Japan.
FAU - Yokoyama, Ayaka
AU  - Yokoyama A
FAU - Miyamoto, Hiroko
AU  - Miyamoto H
FAU - Igarashi, Manabu
AU  - Igarashi M
FAU - Kishida, Noriko
AU  - Kishida N
FAU - Matsuno, Keita
AU  - Matsuno K
FAU - Marzi, Andrea
AU  - Marzi A
FAU - Feldmann, Heinz
AU  - Feldmann H
FAU - Ito, Kimihito
AU  - Ito K
FAU - Saijo, Masayuki
AU  - Saijo M
FAU - Takada, Ayato
AU  - Takada A
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100922
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - *Antigens, Viral/genetics
MH  - Cross Reactions
MH  - Ebolavirus/genetics/immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Filoviridae/immunology/*isolation & purification
MH  - Filoviridae Infections/*diagnosis/immunology/*veterinary
MH  - Haplorhini
MH  - Humans
MH  - Immunoglobulin G/blood
MH  - Immunoglobulin M/blood
MH  - Marburgvirus/genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Primates
MH  - Recombinant Proteins/genetics
MH  - Sensitivity and Specificity
MH  - Virology/*methods
PMC - PMC2976089
EDAT- 2010/09/24 06:00
MHDA- 2011/02/10 06:00
PMCR- 2011/05/01
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2011/02/10 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - CVI.00170-10 [pii]
AID - 0170-10 [pii]
AID - 10.1128/CVI.00170-10 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2010 Nov;17(11):1723-8. doi: 10.1128/CVI.00170-10. Epub 
      2010 Sep 22.