PMID- 20861438
OWN - NLM
STAT- MEDLINE
DCOM- 20110509
LR  - 20220309
IS  - 1522-1598 (Electronic)
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 104
IP  - 6
DP  - 2010 Dec
TI  - Cholinergic modulation of neuronal excitability in the accessory olfactory bulb.
PG  - 2963-74
LID - 10.1152/jn.00446.2010 [doi]
AB  - The accessory olfactory bulb (AOB), the first relay of chemosensory information 
      in the Vomeronasal system, receives extensive cholinergic innervation from the 
      basal forebrain. Cholinergic modulation of neuronal activity in the olfactory 
      bulb has been hypothesized to play an important role in olfactory processing; 
      however, little is known about the cellular actions of acetylcholine (ACh) within 
      the AOB. Here using in vitro slice preparation, we show that muscarinic 
      acetylcholine receptor (mAChR) activation increases neuronal excitability of 
      granule and mitral/tufted cells (GCs and MCs) in the AOB. Activation of mAChRs 
      increased excitability of GCs by three distinct mechanisms: induction of a 
      long-lasting depolarization, activation of a slow afterdepolarization (sADP), and 
      an increase in excitatory glutamatergic input due to MC depolarization. The 
      depolarization and sADP were elicited by the selective agonist 
      4-[[[(3-chlorophenyl)amino]carbonyl]oxy]-N,N,N-trimethyl-2-butyn-1-aminium 
      chloride (100 muM) and blocked by low concentrations of pirenzepine (300 nM), 
      indicating that they result from activation of M1-like mAChRs. In contrast, 
      cholinergic stimulation increased the excitability of MCs via recruitment of 
      nicotinic AChRs (nAChRs) and M1-like mAChRs. Submaximal activation of these 
      receptors, however, decreased the excitability of MCs. Surprisingly, we found 
      that unlike GCs in the main olfactory bulb, GCs in the AOB are excited by mAChR 
      activation in young postnatal neurons, suggesting marked differences in 
      cholinergic regulation of development between these two regions of the olfactory 
      bulb.
FAU - Smith, Richard S
AU  - Smith RS
AD  - Dept. of Biology, Bioscience Research Bldg. R-1239, University of Maryland, 
      College Park, MD 20742, USA.
FAU - Araneda, Ricardo C
AU  - Araneda RC
LA  - eng
GR  - R01 DC009817/DC/NIDCD NIH HHS/United States
GR  - DC RO1-DC-009817/DC/NIDCD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100922
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Muscarinic Agonists)
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (Nicotinic Agonists)
RN  - 0 (Nicotinic Antagonists)
RN  - 0 (Receptor, Muscarinic M1)
RN  - 0 (Receptors, Nicotinic)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/*physiology
MH  - Action Potentials/drug effects
MH  - Age Factors
MH  - Animals
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscarinic Agonists/pharmacology
MH  - Muscarinic Antagonists/pharmacology
MH  - Neurons/*drug effects/physiology
MH  - Nicotinic Agonists/pharmacology
MH  - Nicotinic Antagonists/pharmacology
MH  - Olfactory Bulb/cytology/growth & development/*physiology
MH  - Receptor, Muscarinic M1/*physiology
MH  - Receptors, Nicotinic/drug effects/physiology
PMC - PMC3007668
EDAT- 2010/09/24 06:00
MHDA- 2011/05/10 06:00
PMCR- 2011/12/01
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2011/05/10 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - jn.00446.2010 [pii]
AID - J00446-10 [pii]
AID - 10.1152/jn.00446.2010 [doi]
PST - ppublish
SO  - J Neurophysiol. 2010 Dec;104(6):2963-74. doi: 10.1152/jn.00446.2010. Epub 2010 
      Sep 22.