PMID- 20861567
OWN - NLM
STAT- MEDLINE
DCOM- 20110217
LR  - 20211020
IS  - 0973-7731 (Electronic)
IS  - 0022-1333 (Linking)
VI  - 89
IP  - 2
DP  - 2010 Aug
TI  - Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in 
      Oreochromis mossambicus, O. urolepis hornorum and their hybrid.
PG  - 163-71
AB  - In this study, classical and molecular cytogenetic analyses were performed in 
      tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O. 
      urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing 
      O. mossambicus female x O. u. hornorum male. An identical karyotype ((2n = 44, NF 
      (total number of chromosomal arms) = 50) was obtained from three examined tilapia 
      samples. Genomic organization analysis of 5S rDNA revealed two different types of 
      5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ 
      hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals 
      on six chromosomes of all the analysed metaphases from the three tilapia samples. 
      Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence 
      signals were observed on three chromosome pairs in all analysed metaphases of the 
      three tilapia samples. The correlation between 45 rDNA localization and nucleolar 
      organizer regions (NORs) was confirmed by silver nitrate staining in tilapia 
      fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were 
      verified by two different colour FISH probes. Briefly, the current data provide 
      an insights for hybridization projects and breeding improvement of tilapias.
FAU - Zhu, Hua Ping
AU  - Zhu HP
AD  - Chinese Academy of Fishery Sciences, Guangzhou 510380, People's Republic of 
      China. mx-lu@163.com
FAU - Lu, Mai Xin
AU  - Lu MX
FAU - Gao, Feng Ying
AU  - Gao FY
FAU - Huang, Zhang Han
AU  - Huang ZH
FAU - Yang, Li Ping
AU  - Yang LP
FAU - Gui, Jian Fang
AU  - Gui JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - J Genet
JT  - Journal of genetics
JID - 2985113R
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - China
MH  - Chromosome Mapping
MH  - Crosses, Genetic
MH  - DNA, Ribosomal/*chemistry/*genetics
MH  - Female
MH  - Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Nucleolus Organizer Region/chemistry
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Tilapia/*genetics
EDAT- 2010/09/24 06:00
MHDA- 2011/02/18 06:00
CRDT- 2010/09/24 06:00
PHST- 2010/09/24 06:00 [entrez]
PHST- 2010/09/24 06:00 [pubmed]
PHST- 2011/02/18 06:00 [medline]
AID - 10.1007/s12041-010-0022-1 [doi]
PST - ppublish
SO  - J Genet. 2010 Aug;89(2):163-71. doi: 10.1007/s12041-010-0022-1.