PMID- 20864089 OWN - NLM STAT- MEDLINE DCOM- 20110419 LR - 20231105 IS - 1879-1506 (Electronic) IS - 0003-9969 (Print) IS - 0003-9969 (Linking) VI - 56 IP - 1 DP - 2011 Jan TI - Local and cardiorenal effects of periodontitis in nitric oxide-deficient hypertensive rats. PG - 41-7 LID - 10.1016/j.archoralbio.2010.08.011 [doi] AB - OBJECTIVE: in this study we have assessed the renal and cardiac consequences of ligature-induced periodontitis in both normotensive and nitric oxide (NO)-deficient (L-NAME-treated) hypertensive rats. MATERIALS AND METHODS: oral L-NAME (or water) treatment was started two weeks prior to induction of periodontitis. Rats were sacrificed 3, 7 or 14 days after ligature placement, and alveolar bone loss was evaluated radiographically. Thiobarbituric reactive species (TBARS; a lipid peroxidation index), protein nitrotyrosine (NT; a marker of protein nitration) and myeloperoxidase activity (MPO; a neutrophil marker) were determined in the heart and kidney. RESULTS: in NO-deficient hypertensive rats, periodontitis-induced alveolar bone loss was significantly diminished. In addition, periodontitis-induced cardiac NT elevation was completely prevented by L-NAME treatment. On the other hand L-NAME treatment enhanced MPO production in both heart and kidneys of rats with periodontitis. No changes due to periodontitis were observed in cardiac or renal TBARS content. CONCLUSIONS: in addition to mediating alveolar bone loss, NO contributes to systemic effects of periodontitis in the heart and kidney. CI - 2010 Elsevier Ltd. All rights reserved. FAU - Herrera, Bruno S AU - Herrera BS AD - Department of Pharmacology, ICB, USP, Sao Paulo, SP, Brazil. FAU - Martins-Porto, Rodrigo AU - Martins-Porto R FAU - Campi, Paula AU - Campi P FAU - Holzhausen, Marinella AU - Holzhausen M FAU - Teixeira, Simone A AU - Teixeira SA FAU - Mendes, Gustavo D AU - Mendes GD FAU - Costa, Soraia K P AU - Costa SK FAU - Gyurko, Robert AU - Gyurko R FAU - Van Dyke, Thomas E AU - Van Dyke TE FAU - Spolidorio, Luis C AU - Spolidorio LC FAU - Muscara, Marcelo N AU - Muscara MN LA - eng GR - DE16933/DE/NIDCR NIH HHS/United States GR - K22 DE014568/DE/NIDCR NIH HHS/United States GR - R01 DE016933/DE/NIDCR NIH HHS/United States GR - DE15566/DE/NIDCR NIH HHS/United States GR - R01 DE015566/DE/NIDCR NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20100922 PL - England TA - Arch Oral Biol JT - Archives of oral biology JID - 0116711 RN - 0 (Enzyme Inhibitors) RN - 0 (Free Radical Scavengers) RN - 0 (Thiobarbituric Acid Reactive Substances) RN - 31C4KY9ESH (Nitric Oxide) RN - 3604-79-3 (3-nitrotyrosine) RN - 42HK56048U (Tyrosine) RN - EC 1.11.1.7 (Peroxidase) RN - EC 1.14.13.39 (Nitric Oxide Synthase) RN - V55S2QJN2X (NG-Nitroarginine Methyl Ester) SB - IM MH - Alveolar Bone Loss/diagnostic imaging/metabolism MH - Animals MH - Disease Models, Animal MH - Enzyme Inhibitors/pharmacology MH - Free Radical Scavengers/*antagonists & inhibitors MH - Heart/drug effects MH - Hypertension/*metabolism MH - Kidney/drug effects/*metabolism MH - Male MH - Myocardium/*metabolism MH - NG-Nitroarginine Methyl Ester/pharmacology MH - Nitric Oxide/*antagonists & inhibitors MH - Nitric Oxide Synthase/antagonists & inhibitors MH - Oxidative Stress/physiology MH - Periodontitis/diagnostic imaging/*metabolism MH - Peroxidase/analysis MH - Radiography MH - Rats MH - Rats, Wistar MH - Thiobarbituric Acid Reactive Substances/analysis MH - Tyrosine/analogs & derivatives/analysis PMC - PMC3359128 MID - NIHMS375970 COIS- Competing interests: The authors declare that they have no competing interests. EDAT- 2010/09/25 06:00 MHDA- 2011/04/20 06:00 PMCR- 2012/05/23 CRDT- 2010/09/25 06:00 PHST- 2010/03/24 00:00 [received] PHST- 2010/08/13 00:00 [revised] PHST- 2010/08/21 00:00 [accepted] PHST- 2010/09/25 06:00 [entrez] PHST- 2010/09/25 06:00 [pubmed] PHST- 2011/04/20 06:00 [medline] PHST- 2012/05/23 00:00 [pmc-release] AID - S0003-9969(10)00253-0 [pii] AID - 10.1016/j.archoralbio.2010.08.011 [doi] PST - ppublish SO - Arch Oral Biol. 2011 Jan;56(1):41-7. doi: 10.1016/j.archoralbio.2010.08.011. Epub 2010 Sep 22.