PMID- 20864634
OWN - NLM
STAT- MEDLINE
DCOM- 20110228
LR  - 20211020
IS  - 1943-7811 (Electronic)
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 12
IP  - 6
DP  - 2010 Nov
TI  - Comparison of KRAS mutation analysis and FISH for detecting pancreatobiliary 
      tract cancer in cytology specimens collected during endoscopic retrograde 
      cholangiopancreatography.
PG  - 780-6
LID - 10.2353/jmoldx.2010.100016 [doi]
AB  - Pancreatobiliary tract strictures result either from malignancies of the biliary 
      tract and pancreas or from nonmalignant etiopathogenesis. The goal of this study 
      was to determine whether KRAS mutations could be identified in residual 
      pancreatobiliary stricture brushings and to compare the performance 
      characteristics of KRAS mutation analysis to cytology and fluorescence in situ 
      hybridization (FISH) for the detection of carcinoma. Residual brushing cytology 
      cell pellets were retrieved from 132 patients with subsequent clinicopathologic 
      follow-up of cholangiocarcinoma (n = 41), pancreatic adenocarcinoma (n = 35), 
      gallbladder cancer (n = 2), and nonmalignant strictures (n = 54). All specimens 
      had a prior cytology and FISH UroVysion results as part of clinical practice. 
      KRAS mutation analysis was performed using the quantitative PCR DxS KRAS Mutation 
      Test Kit. KRAS mutation analysis was successful in 130 of 132 specimens. KRAS 
      mutations and polysomic (ie, positive) FISH results were identified in 24 (69%) 
      and 22 (63%) pancreatic adenocarcinoma specimens, respectively, with a combined 
      sensitivity of 86% (30/35). KRAS mutations and polysomic FISH results were 
      identified in 12 (29%) and 17 (41%) cholangiocarcinoma specimens, with a combined 
      sensitivity of 54% (22/41). KRAS mutations were identified in two patients with 
      primary sclerosing cholangitis, and benign follow-up. Residual cytology specimens 
      can be used to detect KRAS mutations by quantitative PCR. Combined KRAS mutation 
      and FISH analysis appear to increase the cancer detection rate in patients with 
      pancreatobiliary strictures.
FAU - Kipp, Benjamin R
AU  - Kipp BR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street 
      Southwest, Rochester, MN 55905, USA.
FAU - Fritcher, Emily G Barr
AU  - Fritcher EG
FAU - Clayton, Amy C
AU  - Clayton AC
FAU - Gores, Gregory J
AU  - Gores GJ
FAU - Roberts, Lewis R
AU  - Roberts LR
FAU - Zhang, Jun
AU  - Zhang J
FAU - Levy, Michael J
AU  - Levy MJ
FAU - Halling, Kevin C
AU  - Halling KC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100923
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (KRAS protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biliary Tract Neoplasms/*diagnosis/genetics/pathology/surgery
MH  - *Cholangiopancreatography, Endoscopic Retrograde
MH  - Cytodiagnosis/*methods
MH  - DNA Mutational Analysis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques
MH  - Mutation
MH  - Pancreatic Neoplasms/*diagnosis/genetics/pathology/surgery
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins p21(ras)
MH  - Young Adult
MH  - ras Proteins/*genetics
PMC - PMC2963905
EDAT- 2010/09/25 06:00
MHDA- 2011/03/01 06:00
PMCR- 2011/11/01
CRDT- 2010/09/25 06:00
PHST- 2010/09/25 06:00 [entrez]
PHST- 2010/09/25 06:00 [pubmed]
PHST- 2011/03/01 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - jmoldx.2010.100016 [pii]
AID - JMDI60128 [pii]
AID - 10.2353/jmoldx.2010.100016 [doi]
PST - ppublish
SO  - J Mol Diagn. 2010 Nov;12(6):780-6. doi: 10.2353/jmoldx.2010.100016. Epub 2010 Sep 
      23.