PMID- 20864677
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20211020
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 177
IP  - 5
DP  - 2010 Nov
TI  - Toll-like receptor (TLR) 2 and TLR9 expressed in trigeminal ganglia are critical 
      to viral control during herpes simplex virus 1 infection.
PG  - 2433-45
LID - 10.2353/ajpath.2010.100121 [doi]
AB  - Herpes simplex virus 1 (HSV-1) is a neurotropic DNA virus that is responsible for 
      several clinical manifestations in humans, including encephalitis. HSV-1 triggers 
      toll-like receptors (TLRs), which elicit cytokine production. Viral 
      multiplication and cytokine expression in C57BL/6 wild-type (WT) mice infected 
      with HSV-1 were evaluated. Virus was found in the trigeminal ganglia (TG), but 
      not in the brains of animals without signs of encephalitis, between 2 and 6 days 
      postinfection (d.p.i.). Cytokine expression in the TG peaked at 5 d.p.i. TLR9-/- 
      and TLR2/9-/- mice were more susceptible to the virus, with 60% and 100% 
      mortality, respectively, as opposed to 10% in the WT and TLR2-/- mice. Increased 
      levels of both CXCL10/IP-10 and CCL2/MCP-1, as well as reduced levels of 
      interferon-gamma and interleukin 1-beta transcripts, measured in both the TG and brains 
      at 5 d.p.i., and the presence of virus in the brain were correlated with total 
      mortality in TLR2/9-/- mice. Cytokine alterations in TLR2/9-/- mice coincided 
      with histopathological changes in their brains, which did not occur in WT and 
      TLR2-/- mice and occurred only slightly in TLR9-/- mouse brain. Increased 
      cellularity, macrophages, CD8 T cells producing interferon-gamma, and expression 
      levels of TLR2 and TLR9 were detected in the TG of WT-infected mice. We 
      hypothesize that HSV-1 infection is controlled by TLR-dependent immune responses 
      in the TG, which prevent HSV-1 encephalitis.
FAU - Lima, Graciela Kunrath
AU  - Lima GK
AD  - Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, Laboratorio de Imunopatologia, CPqRR/FIOCRUZ. Av. 
      Augusto de Lima, 1715. CEP: 30.190-002 Belo Horizonte, MG, Brazil.
FAU - Zolini, Guilherme Pimenta
AU  - Zolini GP
FAU - Mansur, Daniel Santos
AU  - Mansur DS
FAU - Freire Lima, Braulio Henrique
AU  - Freire Lima BH
FAU - Wischhoff, Uschi
AU  - Wischhoff U
FAU - Astigarraga, Ruiz Gerhardt
AU  - Astigarraga RG
FAU - Dias, Marcela Franca
AU  - Dias MF
FAU - das Gracas Almeida Silva, Mariana
AU  - das Gracas Almeida Silva M
FAU - Bela, Samantha Ribeiro
AU  - Bela SR
FAU - do Valle Antonelli, Lis Ribeiro
AU  - do Valle Antonelli LR
FAU - Arantes, Rosa Maria
AU  - Arantes RM
FAU - Gazzinelli, Ricardo Tostes
AU  - Gazzinelli RT
FAU - Bafica, Andre
AU  - Bafica A
FAU - Kroon, Erna Geessien
AU  - Kroon EG
FAU - Campos, Marco Antonio
AU  - Campos MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100923
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 9)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Brain/immunology/pathology/virology
MH  - Cell Line
MH  - Chemokines/blood/immunology
MH  - Cytokines/blood/immunology
MH  - Herpes Simplex/*immunology/*virology
MH  - Herpesvirus 1, Human/*immunology/pathogenicity
MH  - Humans
MH  - Interleukin-12 Subunit p40/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Toll-Like Receptor 2/genetics/*immunology
MH  - Toll-Like Receptor 9/genetics/*immunology
MH  - Trigeminal Ganglion/*immunology/*virology
MH  - Tumor Necrosis Factor-alpha/immunology
PMC - PMC2966801
EDAT- 2010/09/25 06:00
MHDA- 2011/03/22 06:00
PMCR- 2011/11/01
CRDT- 2010/09/25 06:00
PHST- 2010/09/25 06:00 [entrez]
PHST- 2010/09/25 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - S0002-9440(10)60295-7 [pii]
AID - 10.2353/ajpath.2010.100121 [doi]
PST - ppublish
SO  - Am J Pathol. 2010 Nov;177(5):2433-45. doi: 10.2353/ajpath.2010.100121. Epub 2010 
      Sep 23.