PMID- 20868644
OWN - NLM
STAT- MEDLINE
DCOM- 20110301
LR  - 20141120
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 408
IP  - 2
DP  - 2011 Jan 15
TI  - Tetrabutylammonium phosphate-assisted separation of multiplex polymerase chain 
      reaction products in non-gel sieving capillary electrophoresis.
PG  - 284-8
LID - 10.1016/j.ab.2010.09.022 [doi]
AB  - A method based on non-gel sieving capillary electrophoresis (NGS-CE) with 
      ultraviolet (UV) detection has been developed for the separation of multiplex 
      polymerase chain reaction (PCR) products of three pathogenic bacteria in which 
      hydroxypropylmethylcellulose was used as the sieving medium and dynamic capillary 
      coating. In the method, an ion pair reagent, tetrabutylammonium phosphate (TBAP), 
      was first used in NGS-CE to improve the detection sensitivities and resolutions 
      of DNA fragments. The interaction of TBAP and DNA was proved using the UV spectra 
      of DNA with and without TBAP. Field-enhanced sample injection was used as an 
      on-line preconcentration method to improve the detection sensitivity. The 
      separation of DNA fragments ranging from 100 to 1000 bp was accomplished in 30 
      min. Three pairs of primers and three PCR products of bacteria were successfully 
      separated in 25 min using the developed method. The intraday relative standard 
      deviations (RSDs) for the migration time and peak area for each PCR product were 
      less than 2.4% (n=5), and the interday RSDs were less than 6.1% (n=15).
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Zhang, Sheng
AU  - Zhang S
AD  - MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, 
      College of Biophotonics, South China Normal University, Guangzhou 510631, China.
FAU - Jiang, Cheng
AU  - Jiang C
FAU - Jia, Li
AU  - Jia L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100922
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 3NXW29V3WO (Hypromellose Derivatives)
RN  - 9004-67-5 (Methylcellulose)
RN  - 9007-49-2 (DNA)
RN  - CBU2X6BBJR (tetrabutylammonium)
SB  - IM
MH  - DNA/*chemistry/isolation & purification
MH  - Electrophoresis, Capillary/*methods
MH  - Escherichia coli/genetics
MH  - Hypromellose Derivatives
MH  - Listeria monocytogenes/genetics
MH  - Methylcellulose/analogs & derivatives/chemistry
MH  - Polymerase Chain Reaction/methods
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - Salmonella enterica/genetics
MH  - Spectrophotometry, Ultraviolet
EDAT- 2010/09/28 06:00
MHDA- 2011/03/02 06:00
CRDT- 2010/09/28 06:00
PHST- 2010/07/09 00:00 [received]
PHST- 2010/09/13 00:00 [revised]
PHST- 2010/09/15 00:00 [accepted]
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2011/03/02 06:00 [medline]
AID - S0003-2697(10)00607-X [pii]
AID - 10.1016/j.ab.2010.09.022 [doi]
PST - ppublish
SO  - Anal Biochem. 2011 Jan 15;408(2):284-8. doi: 10.1016/j.ab.2010.09.022. Epub 2010 
      Sep 22.