PMID- 20869753
OWN - NLM
STAT- MEDLINE
DCOM- 20101213
LR  - 20161125
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 408
IP  - 23
DP  - 2010 Nov 1
TI  - A microarray data analysis method to evaluate the impact of contaminants on wild 
      animals.
PG  - 5824-7
LID - 10.1016/j.scitotenv.2010.08.065 [doi]
AB  - Here we propose a novel microarray data analysis method applicable to evaluation 
      of the chemical effects on wild animals. First, we analyzed correlations between 
      log-transformed hepatic 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent 
      (TEQ) levels and probe signals detected in wild cormorant liver to screen 
      contaminant-responsive genes. Second, principal component analysis (PCA) was 
      conducted using the screened probes. Third, these probes were divided into two 
      groups based on our PCA result. Finally, we calculated Euclidian distance of 
      signals, which is equivalent to variance of gene expressions, in each probe set, 
      and analyzed the relationship between log-transformed hepatic TEQ levels and 
      Euclidian distances. A probe set whereby the calculated Euclidian distance was 
      positively correlated with TEQ levels, could indicate genes that were directly 
      affected by dioxins or other persistent organic pollutants (POPs), hence they can 
      be used as biomarkers. By contrast, there were a number of probes whereby the 
      Euclidian distance was negatively correlated with TEQ levels. In the latter probe 
      group, the smaller Euclidian distances in highly contaminated individuals could 
      point to changes in physiological activities of wild cormorants. Therefore, our 
      microarray data analysis method will provide new insights into POPs-responsive 
      genes in field-collected samples for toxicogenomics studies.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Nakayama, Kei
AU  - Nakayama K
AD  - Center for Marine Environmental Studies (CMES), Ehime University, 2-5 Bunkyo-cho, 
      Matsuyama 790-8577, Japan. kei-n@agr.ehime-u.ac.jp
FAU - Handoh, Itsuki C
AU  - Handoh IC
FAU - Kitamura, Shin-Ichi
AU  - Kitamura S
FAU - Kim, Eun-Young
AU  - Kim EY
FAU - Iwata, Hisato
AU  - Iwata H
FAU - Tanabe, Shinsuke
AU  - Tanabe S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100924
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
SB  - IM
MH  - Animals
MH  - Birds/genetics/*metabolism
MH  - Environmental Monitoring/*methods
MH  - Environmental Pollutants/*toxicity
MH  - Gene Expression/drug effects
MH  - Gene Expression Profiling/*methods
MH  - Liver/metabolism
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Polychlorinated Dibenzodioxins/*toxicity
EDAT- 2010/09/28 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/09/28 06:00
PHST- 2010/04/26 00:00 [received]
PHST- 2010/08/30 00:00 [revised]
PHST- 2010/08/31 00:00 [accepted]
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0048-9697(10)00938-1 [pii]
AID - 10.1016/j.scitotenv.2010.08.065 [doi]
PST - ppublish
SO  - Sci Total Environ. 2010 Nov 1;408(23):5824-7. doi: 
      10.1016/j.scitotenv.2010.08.065. Epub 2010 Sep 24.