PMID- 20870577
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20111012
IS  - 0003-3898 (Print)
IS  - 0003-3898 (Linking)
VI  - 68
IP  - 5
DP  - 2010 Sep-Oct
TI  - [Assessment of the interest of using confirmatory test in ELISA with use of high 
      concentration of heparin-induced thrombocytopenia].
PG  - 555-60
LID - 10.1684/abc.2010.0465 [doi]
AB  - Heparin-induced thrombocytopenia (HIT) is a severe complication of heparin 
      therapy caused by an antibody against heparin/Platelet Factor 4 (PF4) complex. It 
      complicates the treatment with unfractionated heparin (UFH). The aim of the study 
      was to assess the interest of using confirmatory test in ELISA with use of high 
      concentration of heparin (100 UI/mL) in patients (n=15) with discordance between 
      platelet activation test and ELISA test. We obtained a rate inhibition higher 
      than 50% in 86% of patients was observed. We conclude that inclusion of the high 
      heparin confirmatory procedure in ELISA demonstrate the dependent heparin 
      character of the antibodies but does not improve diagnostic specificity. In 
      addiction a 2-fold increase in final test cost is observed.
FAU - Rissoul, Karima
AU  - Rissoul K
AD  - Laboratoire d'hemostase, Hopital Edouard Herriot, Lyon, France. krm24@hotmail.com
FAU - Madani, Mouhcine
AU  - Madani M
FAU - Nougier, Christophe
AU  - Nougier C
FAU - Rugeri, Lucia
AU  - Rugeri L
FAU - Negrier, Claude
AU  - Negrier C
LA  - fre
PT  - English Abstract
PT  - Journal Article
PT  - Retracted Publication
TT  - Evaluation de l'interet du test de confirmation avec une forte concentration 
      d'heparine en Elisa dans la thrombopenie induite par l'heparine.
PL  - France
TA  - Ann Biol Clin (Paris)
JT  - Annales de biologie clinique
JID - 2984690R
RN  - 0 (Antibodies)
RN  - 9005-49-6 (Heparin)
SB  - IM
RIN - Ann Biol Clin (Paris). 2010 Nov-Dec;68(6):740. PMID: 21972456
MH  - Antibodies/blood
MH  - Costs and Cost Analysis
MH  - Enzyme-Linked Immunosorbent Assay/*methods/standards
MH  - Heparin/*adverse effects/blood
MH  - Humans
MH  - Platelet Activation/drug effects
MH  - Sensitivity and Specificity
MH  - Thrombocytopenia/*chemically induced/*diagnosis/economics
EDAT- 2010/09/28 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/09/28 06:00
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - abc.2010.0465 [pii]
AID - 10.1684/abc.2010.0465 [doi]
PST - ppublish
SO  - Ann Biol Clin (Paris). 2010 Sep-Oct;68(5):555-60. doi: 10.1684/abc.2010.0465.