PMID- 20871863
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20240323
IS  - 1687-9198 (Electronic)
IS  - 1687-918X (Print)
VI  - 2011
DP  - 2011
TI  - Quantification of Persistence of Escherichia coli O157:H7 in Contrasting Soils.
LID - 421379 [pii]
LID - 10.1155/2011/421379 [doi]
AB  - Persistence of Escherichia coli (E. coli) O157:H7 in the environment is a major 
      concern to vegetable and fruit growers where farms and livestock production are 
      in close proximity. The objectives were to determine the effects of preplant 
      fumigation treatment on the survival of E. coli O157:H7 in two soils and the 
      effects of indigenous bacterial populations on the survival of this pathogen. 
      Real-time PCR and plate counts were used to quantify the survival of E. coli 
      O157:H7 in two contrasting soils after fumigation with methyl bromide (MeBr) and 
      methyl iodide (MeI). Ten days after fumigation, E. coli O157:H7 counts were 
      significantly lower (P = .0001) in fumigated soils than in the non-fumigated. 
      Direct comparison between MeBr and MeI within each soil indicated that these two 
      fumigants showed similar impacts on E. coli O157:H7 survival. Microbial species 
      diversity as determined by DGGE was significantly higher in clay soil than sandy 
      soil and this resulted in higher initial decline in population in clay soil than 
      in sandy soil. This study shows that if soil is contaminated with E. coli 
      O157:H7, fumigation alone may not eliminate the pathogen, but may cause decrease 
      in microbial diversity which may enhance the survival of the pathogen.
FAU - Ibekwe, A Mark
AU  - Ibekwe AM
AD  - USDA-ARS, U.S. Salinity Laboratory, 450 W-Big springs Roael, Riverside, CA 92507, 
      USA.
FAU - Papiernik, Sharon K
AU  - Papiernik SK
FAU - Grieve, Catherine M
AU  - Grieve CM
FAU - Yang, Ching-Hong
AU  - Yang CH
LA  - eng
PT  - Journal Article
DEP - 20100908
PL  - Egypt
TA  - Int J Microbiol
JT  - International journal of microbiology
JID - 101516125
PMC - PMC2943103
EDAT- 2010/09/28 06:00
MHDA- 2010/09/28 06:01
PMCR- 2010/09/08
CRDT- 2010/09/28 06:00
PHST- 2010/05/16 00:00 [received]
PHST- 2010/06/29 00:00 [revised]
PHST- 2010/07/09 00:00 [accepted]
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2010/09/28 06:01 [medline]
PHST- 2010/09/08 00:00 [pmc-release]
AID - 421379 [pii]
AID - 10.1155/2011/421379 [doi]
PST - ppublish
SO  - Int J Microbiol. 2011;2011:421379. doi: 10.1155/2011/421379. Epub 2010 Sep 8.