PMID- 20872080
OWN - NLM
STAT- MEDLINE
DCOM- 20110201
LR  - 20211020
IS  - 1661-4917 (Electronic)
IS  - 0004-069X (Print)
IS  - 0004-069X (Linking)
VI  - 58
IP  - 6
DP  - 2010 Dec
TI  - Detection of beta-herpesviruses in Polish adult cord blood stem cell recipients by 
      real-time PCR: single centre study.
PG  - 467-72
LID - 10.1007/s00005-010-0096-8 [doi]
AB  - Umbilical cord blood transplantation (UCBT) is known to be associated with 
      increased risk of infections, compared to bone marrow or peripheral blood stem 
      cell transplantation. In viral diseases for which specific treatment is 
      available, real-time PCR assays are reliable diagnostic tools for timely 
      initiation of appropriate therapy and for rapid assessment of the efficacy of 
      antiviral treatment strategies. A retrospective review of samples from a group of 
      seven adult cord blood stem cell recipients was made. Serum samples taken up to 
      180 days after transplantation were examined with quantitative real-time PCR for 
      measurement of viral load (CMV, HHV-6, and HHV-7). Cytomegalovirus (CMV) DNA was 
      detected in samples taken from four patients (57%) in the period of 20-80 days 
      after transplantation. Products of amplification of human herpesvirus 6 (HHV-6) 
      DNA were found in samples taken between days 25 and 37 following UCBT from only 
      one patient (14%). On the other hand, the majority of patients (n = 6, 86%) had 
      HHV-7 DNA detected in the period 15-58 days after transplantation. Co-infection 
      with HHV-7 was demonstrated at onset of all episodes of microbiologically 
      confirmed CMV or HHV-6 infection. Our observations indicate that real-time PCR is 
      not only useful for monitoring herpesviral infections in transplant recipients, 
      but is also a powerful method for clarifying the relationships between the viral 
      load and clinical symptoms. Further investigation with a much larger group of 
      patients will be needed to confirm these observations and translate them into a 
      clinical approach.
FAU - Dzieciatkowski, Tomasz
AU  - Dzieciatkowski T
AD  - Department of Medical Microbiology, Medical University of Warsaw, Chalubinskiego 
      5, 02-004 Warsaw, Poland. dzieciatkowski@wp.pl
FAU - Przybylski, Maciej
AU  - Przybylski M
FAU - Basak, Grzegorz Wladyslaw
AU  - Basak GW
FAU - Torosian, Tigran
AU  - Torosian T
FAU - Tomaszewska, Agnieszka
AU  - Tomaszewska A
FAU - Jedrzejczak, Wieslaw Wiktor
AU  - Jedrzejczak WW
FAU - Mlynarczyk, Grazyna
AU  - Mlynarczyk G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100925
PL  - Poland
TA  - Arch Immunol Ther Exp (Warsz)
JT  - Archivum immunologiae et therapiae experimentalis
JID - 0114365
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/blood
MH  - Cord Blood Stem Cell Transplantation/*adverse effects/mortality
MH  - Cytomegalovirus/genetics/immunology/*isolation & purification
MH  - Cytomegalovirus Infections/*diagnosis/mortality/virology
MH  - DNA, Viral/*isolation & purification
MH  - Female
MH  - Herpesvirus 6, Human/genetics/immunology/*isolation & purification
MH  - Herpesvirus 7, Human/genetics/immunology/*isolation & purification
MH  - Humans
MH  - Male
MH  - Poland
MH  - *Polymerase Chain Reaction
MH  - Retrospective Studies
MH  - Roseolovirus Infections/*diagnosis/mortality/virology
MH  - Time Factors
MH  - Viral Load
MH  - Young Adult
PMC - PMC7095810
EDAT- 2010/09/28 06:00
MHDA- 2011/02/02 06:00
PMCR- 2020/03/25
CRDT- 2010/09/28 06:00
PHST- 2009/11/16 00:00 [received]
PHST- 2010/03/01 00:00 [accepted]
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2011/02/02 06:00 [medline]
PHST- 2020/03/25 00:00 [pmc-release]
AID - 96 [pii]
AID - 10.1007/s00005-010-0096-8 [doi]
PST - ppublish
SO  - Arch Immunol Ther Exp (Warsz). 2010 Dec;58(6):467-72. doi: 
      10.1007/s00005-010-0096-8. Epub 2010 Sep 25.