PMID- 20872390
OWN - NLM
STAT- MEDLINE
DCOM- 20110211
LR  - 20181201
IS  - 1439-7633 (Electronic)
IS  - 1439-4227 (Linking)
VI  - 11
IP  - 16
DP  - 2010 Nov 2
TI  - Identification of IgE binding to Api g 1-derived peptides.
PG  - 2283-93
LID - 10.1002/cbic.201000322 [doi]
AB  - Celery is a frequent cause of food allergy in pollen-sensitized patients and can 
      induce severe allergic reactions. Clinical symptoms cannot be predicted by skin 
      prick tests (SPTs) or by determining allergen-specific immunoglobulin E (IgE). 
      Our aim was to identify specific IgE binding peptides by using an array 
      technique. For our study, the sera of 21 patients with positive double-blind, 
      placebo-controlled food challenge (DBPCFC) to celery, as well as the sera of 17 
      healthy patients were used. Additionally, all patients underwent skin tests along 
      with determinations of specific IgE binding. The major allergen of celery 
      Api g 1.0101 (Apium graveolens) was synthesized as an array of overlapping 
      peptides and probed with the patients' sera. We developed an improved immunoassay 
      protocol by investigating peptide lengths, peptide densities, incubation 
      parameters, and readout systems, which could influence IgE binding. Sera of 
      celery-allergic patients showed binding to three distinct regions of 
      Api g 1.0101. The region including amino acids 100 to 126 of Api g 1.0101 is the 
      most important region for IgE binding. This region caused a fivefold higher 
      binding of IgE from the sera of celery-allergic patients compared to those of 
      healthy individuals. In particular, one peptide (VLVPTADGGSIC) was recognized by 
      all sera of celery-allergic patients. In contrast, no binding to this peptide was 
      detected in sera of the healthy controls. Our improved assay strategy allows us 
      to distinguish between celery-allergic and healthy individuals, but needs to be 
      explored in a larger cohort of well-defined patients.
FAU - Ruppel, Elvira
AU  - Ruppel E
AD  - Allergie-Centrum-Charite, Klinik fur Dermatologie und Allergologie, 
      Charite-Universitatsmedizin Berlin, Berlin, Germany.
FAU - Ay, Bernhard
AU  - Ay B
FAU - Boisguerin, Prisca
AU  - Boisguerin P
FAU - Dolle, Sabine
AU  - Dolle S
FAU - Worm, Margitta
AU  - Worm M
FAU - Volkmer, Rudolf
AU  - Volkmer R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Chembiochem
JT  - Chembiochem : a European journal of chemical biology
JID - 100937360
RN  - 0 (Antigens, Plant)
RN  - 0 (Api g 1 protein, Apium graveolens)
RN  - 0 (Peptides)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Antigens, Plant/chemistry/*immunology
MH  - Apium/*immunology
MH  - Food Hypersensitivity/diagnosis/*immunology
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin E/blood/*immunology
MH  - Middle Aged
MH  - Peptides/*immunology
MH  - Plant Proteins/chemistry/*immunology
MH  - Protein Array Analysis
MH  - Protein Binding
MH  - Skin Tests
EDAT- 2010/09/28 06:00
MHDA- 2011/02/12 06:00
CRDT- 2010/09/28 06:00
PHST- 2010/09/28 06:00 [entrez]
PHST- 2010/09/28 06:00 [pubmed]
PHST- 2011/02/12 06:00 [medline]
AID - 10.1002/cbic.201000322 [doi]
PST - ppublish
SO  - Chembiochem. 2010 Nov 2;11(16):2283-93. doi: 10.1002/cbic.201000322.