PMID- 20873582
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 6
DP  - 2010 Jun
TI  - [Determination of zearalenone and related mycotoxins in grain and its products by 
      solid-phase extraction coupled with ultra performance liquid 
      chromatography-tandem mass spectrometry].
PG  - 601-7
AB  - A method was established for the determination of 6 zearalenonic compounds 
      (alpha-zearalanol, beta-zearalanol, alpha-zearalenol, beta-zearalenol, 
      zearalanone and zearalenone) in grain and its products based on solid-phase 
      extraction coupled with ultra performance liquid chromatography-tandem mass 
      spectrometry (UPLC-MS/MS). The samples was extracted by 84% (v/v) 
      acetonitrile-water solution and further purified by an ENVI-Carb graphite carbon 
      black (GCB) cartridge, which was eluted by 6 mL dichloromethane-methanol (7:3, 
      v/v) solution. The target compounds were assayed by UPLC-MS/MS. The 
      chromatographic separation was performed on an ACQUITY UPLC BEH C18 column with 
      gradient elution using acetonitrile and water as mobile phases. The mass 
      spectrometric acquisitions were carried out by means of multiple reaction 
      monitoring (MRM) in electrospray negative ionization mode. The good linearities 
      (R2 > 0.99) were achieved for the 6 compounds over the range of 0.1-50 microg/L 
      based on the internal standard calibration of a-zearalenol-d4. The detection 
      limits of the method were 0.1-0.2 microg/kg. The mean recoveries of the 6 target 
      compounds (spiked at three concentration levels) ranged from 79.9% to 104.0%, 
      with the relative standard deviations (RSDs) no more than 10%. It has been 
      applied in the analysis of grain and related products taken from Beijing. As a 
      result, zearalenone presented a highest detectable frequency, with a 
      concentration range of 0.42-220.7 microg/kg. In addition, alpha-zearalenol and 
      beta-zearalenol were also detected in this survey. This proposed method is 
      simple, sensitive, reproducible, and complied with the regulations for the 
      determination of trace contaminants residues in food matrices.
FAU - Meng, Juan
AU  - Meng J
AD  - Beijing Center for Disease Control & Prevention, Beijing 100013, China.
FAU - Zhang, Jing
AU  - Zhang J
FAU - Zhang, Nan
AU  - Zhang N
FAU - Shi, Jiachen
AU  - Shi J
FAU - Shao, Bing
AU  - Shao B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Mycotoxins)
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Edible Grain/*chemistry
MH  - Food Contamination/analysis
MH  - Mycotoxins/*analysis
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Zearalenone/*analysis
EDAT- 2010/09/30 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/09/30 06:00
PHST- 2010/09/30 06:00 [entrez]
PHST- 2010/09/30 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00601 [doi]
PST - ppublish
SO  - Se Pu. 2010 Jun;28(6):601-7. doi: 10.3724/sp.j.1123.2010.00601.