PMID- 20873584
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20231213
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 6
DP  - 2010 Jun
TI  - [Simultaneous analysis of twenty free amino acids in tobacco using liquid 
      chromatography-electrospray ionization/ion trap tandem mass spectrometry].
PG  - 615-22
AB  - A method for simultaneous analysis of 20 free amino acids in tobacco was 
      developed using liquid chromatography-electrospray ionization/ion trap tandem 
      mass spectrometry (LC-ESI-IT-MS/MS). Tobacco samples were extracted and filtered, 
      and analyzed without derivatization or solid phase extraction. The LC separation 
      was carried on a reversed-phase HyPURITY C18 column (200 mm x 2.1 mm, 5 microm) 
      with the mobile phase of 1% (v/v) acetonitrile/water solution containing 0.1% 
      nonafluoropentanoic acid and 90% acetonitrile/water solution containing 0.1% 
      nonafluoropentanoic acid in gradient mode. The results showed that the limits of 
      detection (LODs) of 20 amino acids were in the range of 0.01-0.05 micromol/L (S/N 
      = 3), the linear correlation coefficients were above 0. 997 7 in all cases, and 
      the relative standard deviations (RSDs) of peak areas of the extracted ion 
      chromatograms were in the range of 0.78%-4.93%. The developed method is of good 
      efficiency, sensitivity and selectivity, and has been successfully applied to 
      determine the free amino acids in varieties of tobacco samples.
FAU - Huang, Yifei
AU  - Huang Y
AD  - Technology Center, China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 
      510145, China. hyfchem@yahoo.com.cn
FAU - Hu, Jing
AU  - Hu J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Amino Acids)
SB  - IM
MH  - Amino Acids/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Nicotiana/*chemistry
EDAT- 2010/09/30 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/09/30 06:00
PHST- 2010/09/30 06:00 [entrez]
PHST- 2010/09/30 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00615 [doi]
PST - ppublish
SO  - Se Pu. 2010 Jun;28(6):615-22. doi: 10.3724/sp.j.1123.2010.00615.