PMID- 20874756
OWN - NLM
STAT- MEDLINE
DCOM- 20101129
LR  - 20131121
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 115
IP  - 5
DP  - 2010 Dec
TI  - Involvement of the 3'-untranslated region of the brain-derived neurotrophic 
      factor gene in activity-dependent mRNA stabilization.
PG  - 1222-33
LID - 10.1111/j.1471-4159.2010.07016.x [doi]
AB  - Although gene transcription is controlled by neuronal activity, little is known 
      about post-transcriptional regulation in neurons. Using cultured neurons, we 
      found that the half-life of immediate-early gene transcripts is prolonged or 
      shortened by membrane depolarization. Focusing on the activity-dependent 
      stabilization of brain-derived neurotrophic factor (BDNF) mRNA, we constructed a 
      series of plasmids, in which the short 3'-untranslated region (3'-UTR) of the 
      BDNF gene was fused to the firefly luciferase gene, and found that the 3'-UTR 
      prevented destabilization of luciferase mRNA through Ca(2+) signals evoked via 
      depolarization. No such prevention was observed with the simian virus 40 late 
      poly(A) site. The pre-mRNA covering the entire short 3'-UTR, where multiple 
      poly(A) sites including novel ones are located, was stabilized. Deletion analyses 
      of 3'-UTR revealed a core region (about 130 bases long) and a complementary 
      region to be responsible for the prevention, well consistent with the formation 
      of an extended stem-loop RNA structure and the production of poly(A) mRNAs. Thus, 
      the mRNA stability is activity-dependently controlled in neurons and distinct 
      regions of the 3'-UTR of BDNF mRNA are involved in stabilizing mRNA in response 
      to Ca(2+) signals, suggesting a primary role of the RNA secondary structure 
      affecting the availability of poly(A) sites in activity-dependent mRNA 
      stabilization.
CI  - (c) 2010 The Authors. Journal of Neurochemistry (c) 2010 International Society for 
      Neurochemistry.
FAU - Fukuchi, Mamoru
AU  - Fukuchi M
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama, Japan.
FAU - Tsuda, Masaaki
AU  - Tsuda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101026
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (brain-derived growth factor)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 76326-31-3 (2-amino-5-phosphopentanoic acid)
RN  - CZ5312222S (Nicardipine)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - 3' Untranslated Regions/*physiology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Signaling/drug effects/genetics
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Dactinomycin/pharmacology
MH  - Embryo, Mammalian
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Gene Expression Regulation/*genetics
MH  - Genes, Immediate-Early/physiology
MH  - Neurons
MH  - Nicardipine/pharmacology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA Precursors/genetics/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transfection/methods
MH  - Valine/analogs & derivatives/pharmacology
EDAT- 2010/09/30 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/09/30 06:00
PHST- 2010/09/30 06:00 [entrez]
PHST- 2010/09/30 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1111/j.1471-4159.2010.07016.x [doi]
PST - ppublish
SO  - J Neurochem. 2010 Dec;115(5):1222-33. doi: 10.1111/j.1471-4159.2010.07016.x. Epub 
      2010 Oct 26.