PMID- 20874812
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20231213
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Linking)
VI  - 11
IP  - 12
DP  - 2010 Dec
TI  - Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde 
      transport.
PG  - 1552-66
LID - 10.1111/j.1600-0854.2010.01123.x [doi]
AB  - The coiled-coil Golgi membrane protein golgin-84 functions as a tethering factor 
      for coat protein I (COPI) vesicles. Protein interaction analyses have revealed 
      that golgin-84 interacts with another tether, the conserved oligomeric Golgi 
      (COG) complex, through its subunit Cog7. Therefore, we explored the function of 
      golgin-84 as the tether for COPI vesicles of intra-Golgi retrograde traffic. 
      First, glycosylic maturation of both plasma membrane (CD44) and lysosomal (lamp1) 
      glycoproteins was distorted in golgin-84 knockdown (KD) cells. The depletion of 
      golgin-84 caused fragmentation of the Golgi with the mislocalization of Golgi 
      resident proteins, resulting in the accumulation of vesicles carrying intra-Golgi 
      soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) 
      and cis-Golgi membrane protein GPP130. Similar observations were obtained by 
      diminution of the COG complex, suggesting a strong correlation between the two 
      tethers. Indeed, COG complex-dependent (CCD) vesicles that accumulate in Cog3 or 
      Cog7 KD cells carried golgin-84. Surprisingly, the interaction between golgin-84 
      and another candidate tethering partner CASP (CDP/cut alternatively spliced 
      product) decreased in Cog3 KD cells. These results indicate that golgin-84 on 
      COPI vesicles interact with the COG complex before SNARE assembly, suggesting 
      that the interaction of golgin-84 with COG plays an important role in the 
      tethering process of intra-Golgi retrograde vesicle traffic.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Sohda, Miwa
AU  - Sohda M
AD  - Division of Oral Biochemistry, Niigata University Graduate School of Medical and 
      Dental Sciences, Chuo-ku, Niigata 951-8514, Japan.
FAU - Misumi, Yoshio
AU  - Misumi Y
FAU - Yamamoto, Akitsugu
AU  - Yamamoto A
FAU - Nakamura, Nobuhiro
AU  - Nakamura N
FAU - Ogata, Shigenori
AU  - Ogata S
FAU - Sakisaka, Shotaro
AU  - Sakisaka S
FAU - Hirose, Shinichi
AU  - Hirose S
FAU - Ikehara, Yukio
AU  - Ikehara Y
FAU - Oda, Kimimitsu
AU  - Oda K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101015
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (CD44 protein, human)
RN  - 0 (COG3 protein, human)
RN  - 0 (COG7 protein, human)
RN  - 0 (GOLGA5 protein, human)
RN  - 0 (Golgi Matrix Proteins)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (LAMP1 protein, human)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (SNARE Proteins)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport/metabolism
MH  - Biological Transport
MH  - Cell Line
MH  - Golgi Apparatus/*metabolism
MH  - Golgi Matrix Proteins
MH  - Humans
MH  - Hyaluronan Receptors
MH  - Lysosomal Membrane Proteins/metabolism
MH  - Membrane Proteins/*metabolism
MH  - Protein Binding
MH  - SNARE Proteins/metabolism
MH  - Vesicular Transport Proteins/metabolism
EDAT- 2010/09/30 06:00
MHDA- 2011/03/15 06:00
CRDT- 2010/09/30 06:00
PHST- 2010/09/30 06:00 [entrez]
PHST- 2010/09/30 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
AID - 10.1111/j.1600-0854.2010.01123.x [doi]
PST - ppublish
SO  - Traffic. 2010 Dec;11(12):1552-66. doi: 10.1111/j.1600-0854.2010.01123.x. Epub 
      2010 Oct 15.