PMID- 20878655
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20101014
IS  - 1613-6829 (Electronic)
IS  - 1613-6810 (Linking)
VI  - 6
IP  - 20
DP  - 2010 Oct 18
TI  - Hybrid polymer-grafted multiwalled carbon nanotubes for in vitro gene delivery.
PG  - 2281-91
LID - 10.1002/smll.201000864 [doi]
AB  - Carbon nanotubes (CNTs) consist of carbon atoms arranged in sheets of graphene 
      rolled up into cylindrical shapes. This class of nanomaterials has attracted 
      attention because of their extraordinary properties, such as high electrical and 
      thermal conductivity. In addition, development in CNT functionalization chemistry 
      has led to an enhanced dispersibility in aqueous physiological media which indeed 
      broadens the spectrum for their potential biological applications including gene 
      delivery. The aim of this study is to determine the capability of different 
      cationic polymer-grafted multiwalled carbon nanotubes (MWNTs) (polymer-g-MWNTs) 
      to efficiently complex and transfer plasmid DNA (pCMV-betaGal) in vitro without 
      promoting cytotoxicity. Carboxylated MWNT is chemically conjugated to the 
      cationic polymers polyethylenimine (PEI), polyallylamine (PAA), or a mixture of 
      the two polymers. In order to explore the potential of these polymer-g-MWNTs as 
      gene delivery systems, we first study their capacity to complex plasmid DNA 
      (pDNA) using agarose gel electrophoresis. Gel migration studies confirm pDNA 
      binding to polymer-g-MWNT with different affinities, highest for PEI-g-MWNT and 
      PEI/PAA-g-CNT constructs. beta-galactosidase expression is assessed in human lung 
      epithelial (A549) cells, and the cytotoxicity is determined by modified LDH assay 
      after 24 h incubation period. Additionally, PEI-g-MWNT and/or PEI/PAA-g-MWNT 
      reveal an improvement in gene expression when compared to the naked pDNA or to 
      the equivalent amounts of PEI polymer alone. Mechanistically, pDNA was delivered 
      by the polymer-g-MWNT constructs via a different pathway compared to those used 
      by polyplexes. In conclusion, polymer-g-MWNTs may be considered in the future as 
      a versatile tool for efficient gene transfer in cancer cells in vitro, provided 
      their toxicological profile is established.
FAU - Nunes, Antonio
AU  - Nunes A
AD  - Nanomedicine Laboratory, Centre for Drug Delivery Research, The School of 
      Pharmacy, University of London, UK.
FAU - Amsharov, Nadja
AU  - Amsharov N
FAU - Guo, Chang
AU  - Guo C
FAU - Van den Bossche, Jeroen
AU  - Van den Bossche J
FAU - Santhosh, Padmanabhan
AU  - Santhosh P
FAU - Karachalios, Theodoros K
AU  - Karachalios TK
FAU - Nitodas, Stephanos F
AU  - Nitodas SF
FAU - Burghard, Marko
AU  - Burghard M
FAU - Kostarelos, Kostas
AU  - Kostarelos K
FAU - Al-Jamal, Khuloud T
AU  - Al-Jamal KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Small
JT  - Small (Weinheim an der Bergstrasse, Germany)
JID - 101235338
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Polyamines)
RN  - 0 (Polymers)
RN  - 30551-89-4 (polyallylamine)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Cell Line, Tumor
MH  - Electrophoresis, Agar Gel
MH  - *Gene Transfer Techniques
MH  - Humans
MH  - Magnetic Resonance Spectroscopy
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Nanomedicine/methods
MH  - Nanotubes, Carbon/*chemistry/ultrastructure
MH  - Plasmids/chemistry/genetics
MH  - Polyamines/chemistry
MH  - Polyethyleneimine/chemistry
MH  - Polymers/*chemistry
MH  - Spectrum Analysis, Raman
EDAT- 2010/09/30 06:00
MHDA- 2011/02/01 06:00
CRDT- 2010/09/30 06:00
PHST- 2010/09/30 06:00 [entrez]
PHST- 2010/09/30 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
AID - 10.1002/smll.201000864 [doi]
PST - ppublish
SO  - Small. 2010 Oct 18;6(20):2281-91. doi: 10.1002/smll.201000864.