PMID- 20881193
OWN - NLM
STAT- MEDLINE
DCOM- 20101112
LR  - 20101021
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 185
IP  - 9
DP  - 2010 Nov 1
TI  - TGF-beta1 accelerates dendritic cell differentiation from common dendritic cell 
      progenitors and directs subset specification toward conventional dendritic cells.
PG  - 5326-35
LID - 10.4049/jimmunol.0903950 [doi]
AB  - Dendritic cells (DCs) in lymphoid tissue comprise conventional DCs (cDCs) and 
      plasmacytoid DCs (pDCs) that develop from common DC progenitors (CDPs). CDPs are 
      Flt3(+)c-kit(int)M-CSFR(+) and reside in bone marrow. In this study, we describe 
      a two-step culture system that recapitulates DC development from 
      c-kit(hi)Flt3(-/lo) multipotent progenitors (MPPs) into CDPs and further into cDC 
      and pDC subsets. MPPs and CDPs are amplified in vitro with Flt3 ligand, stem cell 
      factor, hyper-IL-6, and insulin-like growth factor-1. The four-factor mixture 
      readily induces self-renewal of MPPs and their progression into CDPs and has no 
      self-renewal activity on CDPs. The amplified CDPs respond to all known DC 
      poietins and generate all lymphoid tissue DCs in vivo and in vitro. Additionally, 
      in vitro CDPs recapitulate the cell surface marker and gene expression profile of 
      in vivo CDPs and possess a DC-primed transcription profile. TGF-beta1 impacts on 
      CDPs and directs their differentiation toward cDCs. Genome-wide gene expression 
      profiling of TGF-beta1-induced genes identified instructive transcription factors 
      for cDC subset specification, such as IFN regulatory factor-4 and RelB. TGF-beta1 
      also induced the transcription factor inhibitor of differentiation/DNA binding 2 
      that suppresses pDC development. Thus, TGF-beta1 directs CDP differentiation into 
      cDCs by inducing both cDC instructive factors and pDC inhibitory factors.
FAU - Felker, Piritta
AU  - Felker P
AD  - Department of Cell Biology, Medical Faculty, Rheinisch-Westfalische Technische 
      Hochschule, Aachen, Germany.
FAU - Sere, Kristin
AU  - Sere K
FAU - Lin, Qiong
AU  - Lin Q
FAU - Becker, Christiane
AU  - Becker C
FAU - Hristov, Mihail
AU  - Hristov M
FAU - Hieronymus, Thomas
AU  - Hieronymus T
FAU - Zenke, Martin
AU  - Zenke M
LA  - eng
SI  - GEO/GSE22432
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100929
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*immunology
MH  - Cell Separation
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Gene Knock-In Techniques
MH  - Hematopoietic Stem Cells/*cytology/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Multipotent Stem Cells/cytology/immunology/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Transforming Growth Factor beta1/*immunology/metabolism
EDAT- 2010/10/01 06:00
MHDA- 2010/11/13 06:00
CRDT- 2010/10/01 06:00
PHST- 2010/10/01 06:00 [entrez]
PHST- 2010/10/01 06:00 [pubmed]
PHST- 2010/11/13 06:00 [medline]
AID - jimmunol.0903950 [pii]
AID - 10.4049/jimmunol.0903950 [doi]
PST - ppublish
SO  - J Immunol. 2010 Nov 1;185(9):5326-35. doi: 10.4049/jimmunol.0903950. Epub 2010 
      Sep 29.