PMID- 20881208
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 116
IP  - 26
DP  - 2010 Dec 23
TI  - Rho-mDia1 pathway is required for adhesion, migration, and T-cell stimulation in 
      dendritic cells.
PG  - 5875-84
LID - 10.1182/blood-2010-01-264150 [doi]
AB  - Dendritic cells (DCs) are essential for the initiation of acquired immune 
      responses through antigen acquisition, migration, maturation, and T-cell 
      stimulation. One of the critical mechanisms in this response is the process actin 
      nucleation and polymerization, which is mediated by several groups of proteins, 
      including mammalian Diaphanous-related formins (mDia). However, the role of mDia 
      in DCs remains unknown. Herein, we examined the role of mDia1 (one of the 
      isoforms of mDia) in DCs. Although the proliferation and maturation of bone 
      marrow-derived DCs were comparable between control C57BL/6 and mDia1-deficient 
      (mDia1(-/-)) mice, adhesion and spreading to cellular matrix were impaired in 
      mDia1(-/-) bone marrow-derived DCs. In addition, fluorescein 
      isothiocyanate-induced cutaneous DC migration to draining lymph nodes in vivo and 
      invasive migration and directional migration to CCL21 in vitro were suppressed in 
      mDia1(-/-) DCs. Moreover, sustained T-cell interaction and T-cell stimulation in 
      lymph nodes were impaired by mDia1 deficiency. Consistent with this, the 
      DC-dependent delayed hypersensitivity response was attenuated by mDia1-deficient 
      DCs. These results suggest that actin polymerization, which is mediated by mDia1, 
      is essential for several aspects of DC-initiated acquired immune responses.
FAU - Tanizaki, Hideaki
AU  - Tanizaki H
AD  - Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto, 
      Japan.
FAU - Egawa, Gyohei
AU  - Egawa G
FAU - Inaba, Kayo
AU  - Inaba K
FAU - Honda, Tetsuya
AU  - Honda T
FAU - Nakajima, Saeko
AU  - Nakajima S
FAU - Moniaga, Catharina Sagita
AU  - Moniaga CS
FAU - Otsuka, Atsushi
AU  - Otsuka A
FAU - Ishizaki, Toshimasa
AU  - Ishizaki T
FAU - Tomura, Michio
AU  - Tomura M
FAU - Watanabe, Takeshi
AU  - Watanabe T
FAU - Miyachi, Yoshiki
AU  - Miyachi Y
FAU - Narumiya, Shuh
AU  - Narumiya S
FAU - Okada, Takaharu
AU  - Okada T
FAU - Kabashima, Kenji
AU  - Kabashima K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100929
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Carrier Proteins)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Diap1 protein, mouse)
RN  - 0 (Formins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carrier Proteins/*physiology
MH  - *Cell Adhesion
MH  - *Cell Movement
MH  - Chemokine CCL21/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Formins
MH  - Lymph Nodes/immunology/metabolism/pathology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*immunology/*metabolism
MH  - rho GTP-Binding Proteins/genetics/*metabolism
EDAT- 2010/10/01 06:00
MHDA- 2011/02/23 06:00
CRDT- 2010/10/01 06:00
PHST- 2010/10/01 06:00 [entrez]
PHST- 2010/10/01 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
AID - S0006-4971(20)34792-3 [pii]
AID - 10.1182/blood-2010-01-264150 [doi]
PST - ppublish
SO  - Blood. 2010 Dec 23;116(26):5875-84. doi: 10.1182/blood-2010-01-264150. Epub 2010 
      Sep 29.