PMID- 20881297
OWN - NLM
STAT- MEDLINE
DCOM- 20110524
LR  - 20211020
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 52
IP  - 3
DP  - 2011 Mar
TI  - Upregulation of mouse genes in HSV-1 latent TG after butyrate treatment 
      implicates the multiple roles of the LAT-ICP0 locus.
PG  - 1770-9
LID - 10.1167/iovs.09-5019 [doi]
AB  - PURPOSE: To determine host response by gene expression in HSV-1 latent trigeminal 
      ganglia (TG) after sodium butyrate (NaBu) treatment. METHODS: Corneas of 
      6-week-old female BALB/c mice were scarified and inoculated with HSV-1 17Syn(+) 
      (high phenotypic reactivator) or its mutant 17DeltaPst(LAT(-)) (low phenotypic 
      reactivator) at 10(4) plaque-forming units/eye. NaBu-induced viral reactivation 
      was by intraperitoneal (IP) administration at postinfection (PI) day 28, followed 
      by euthanasia after 1 hour. NaBu-treated, uninfected mice served as the control. 
      The resultant labeled cRNA from TG isolated total RNA was hybridized to gene 
      microarray chips containing 14,000 mouse genes. Quantitative real-time PCR was 
      performed to confirm gene expression. RESULTS: Differential induction of gene 
      expression between 17Syn(+) and its mutant 17DeltaPst(LAT(-)) was designated as 
      NaBu-induced gene expression and yielded significant upregulation of 2- to 
      16-fold of 0.4% (56/14,000) host genes probed, comprising mainly nucleosome 
      assembly and binding, central nervous system structural activity, hormonal 
      activity, and signaling activity. Approximately 0.2% (24/14,000) of the host 
      genes, mainly of the same functional categories were downregulated 3- to 11-fold. 
      Immune activity was minor in comparison to our reports on gene expression during 
      latency and heat stress induction. Euchromatin analysis revealed that the 
      LAT-ICP0 locus is amenable to the effects of NaBu. Histone activity was detected 
      by early transcription of histone cluster 2 H2be (Hist2h2be). CONCLUSIONS 
      NaBu-induced reactivation of HSV-1 is twofold: drug action involving significant 
      moderation of specific host epigenetic changes and failure to elicit or suppress 
      immune activity at the early time point of 1 hour.
FAU - Clement, Christian
AU  - Clement C
AD  - Department of Ophthalmology, LSU Eye Center, Louisiana State University Health 
      Sciences Center, New Orleans, Louisiana, USA.
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
FAU - Kumar, Manish
AU  - Kumar M
FAU - Foster, Timothy P
AU  - Foster TP
FAU - Thompson, Hilary W
AU  - Thompson HW
FAU - Hill, James M
AU  - Hill JM
LA  - eng
GR  - P30 EY002377/EY/NEI NIH HHS/United States
GR  - R01 EY006311/EY/NEI NIH HHS/United States
GR  - EY02377/EY/NEI NIH HHS/United States
GR  - EY006311/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110328
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Butyrates)
RN  - 0 (DNA, Viral)
RN  - 0 (Histones)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (Nucleosomes)
RN  - 0 (latency associated transcript, herpes simplex virus-1)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.3.2.27 (Vmw110 protein, Human herpesvirus 1)
SB  - IM
MH  - Animals
MH  - Butyrates/*pharmacology
MH  - Cornea/innervation
MH  - DNA Copy Number Variations
MH  - DNA, Viral/genetics
MH  - Female
MH  - Gene Expression Regulation, Viral/*drug effects
MH  - Herpesvirus 1, Human/*genetics/physiology
MH  - Histones/metabolism
MH  - Immediate-Early Proteins/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*physiology
MH  - Nucleosomes/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Trigeminal Ganglion/*virology
MH  - Ubiquitin-Protein Ligases/*physiology
MH  - Up-Regulation
MH  - Virus Activation/drug effects
MH  - Virus Latency/*physiology
PMC - PMC3101669
EDAT- 2010/10/01 06:00
MHDA- 2011/05/25 06:00
PMCR- 2012/03/01
CRDT- 2010/10/01 06:00
PHST- 2010/10/01 06:00 [entrez]
PHST- 2010/10/01 06:00 [pubmed]
PHST- 2011/05/25 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - iovs.09-5019 [pii]
AID - 09-5019 [pii]
AID - 10.1167/iovs.09-5019 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2011 Mar 28;52(3):1770-9. doi: 10.1167/iovs.09-5019. 
      Print 2011 Mar.