PMID- 20881413
OWN - NLM
STAT- MEDLINE
DCOM- 20110127
LR  - 20131121
IS  - 1423-0151 (Electronic)
IS  - 1011-7571 (Linking)
VI  - 19
IP  - 6
DP  - 2010
TI  - Calpain-10 gene polymorphism in type 2 diabetes mellitus patients in the Gaza 
      Strip.
PG  - 457-62
LID - 10.1159/000320304 [doi]
AB  - OBJECTIVE: To examine the role of calpain-10 SNP-44, -43, -63 and del/ins-19 in 
      genetic susceptibility to type 2 diabetes mellitus (T2DM) and associations with 
      triglycerides and total cholesterol in a group of subjects residing in the Gaza 
      Strip. SUBJECTS AND METHODS: Ninety-six individuals were examined: 48 T2DM 
      patients and 48 controls. The groups were genotyped for calpain-10 SNP-44, -43, 
      -63, and del/ins-19. Mutagenically separated polymerase chain reaction was used 
      to examine SNP-44; del/ins-19 was examined by electrophoresis of the PCR product 
      on agarose gel, while the restriction fragment length polymorphism method was 
      used for SNP-43 and -63. RESULTS: There was evidence that the C allele at SNP-44 
      played a possible role in susceptibility to T2DM (p = 0.01). T2DM patients with 
      G/A genotype were found to have higher levels of total cholesterol in comparison 
      to those homozygous for allele 1 (G/G) in SNP-43. Total cholesterol levels 
      increased in T2DM patients who are homozygous for del/ins-19 allele 2, in T2DM 
      patients with the 121/221 haplotype combination, and in control subjects with the 
      haplotype combination 111/121. CONCLUSION: SNP-44 polymorphism of the calpain-10 
      gene has a significant association with T2DM patients in the Gaza strip. Certain 
      polymorphisms of calpain-10 also have associations with the levels of total 
      cholesterol in both T2DM patients and controls.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Zaharna, Mazen M
AU  - Zaharna MM
AD  - Department of Medical Technology, Islamic University of Gaza, Gaza City, 
      Palestine. mzaharna@iugaza.edu.ps
FAU - Abed, Abdalla A
AU  - Abed AA
FAU - Sharif, Fadel A
AU  - Sharif FA
LA  - eng
PT  - Journal Article
DEP - 20100928
PL  - Switzerland
TA  - Med Princ Pract
JT  - Medical principles and practice : international journal of the Kuwait University, 
      Health Science Centre
JID - 8901334
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.4.22.- (Calpain)
RN  - EC 3.4.22.- (calpain 10)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Arabs
MH  - Calpain/*genetics
MH  - Cholesterol/blood
MH  - Diabetes Mellitus, Type 2/ethnology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genome-Wide Association Study
MH  - Haplotypes
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Middle East/epidemiology
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Single Nucleotide
MH  - Triglycerides/blood
EDAT- 2010/10/01 06:00
MHDA- 2011/01/29 06:00
CRDT- 2010/10/01 06:00
PHST- 2009/02/15 00:00 [received]
PHST- 2009/08/13 00:00 [accepted]
PHST- 2010/10/01 06:00 [entrez]
PHST- 2010/10/01 06:00 [pubmed]
PHST- 2011/01/29 06:00 [medline]
AID - 000320304 [pii]
AID - 10.1159/000320304 [doi]
PST - ppublish
SO  - Med Princ Pract. 2010;19(6):457-62. doi: 10.1159/000320304. Epub 2010 Sep 28.