PMID- 20883122
OWN - NLM
STAT- MEDLINE
DCOM- 20110228
LR  - 20101116
IS  - 1939-6376 (Electronic)
IS  - 1939-6368 (Linking)
VI  - 56
IP  - 6
DP  - 2010 Dec
TI  - Disruption of telomere-telomere interactions associated with DNA damage in human 
      spermatozoa.
PG  - 407-12
LID - 10.3109/19396368.2010.502587 [doi]
AB  - Telomeres play a fundamental role in the organization of the sperm nucleus 
      resulting in the looped chromosome configuration and non-random positioning of 
      chromosomes. Telomeres localize in the nuclear periphery and interact dynamically 
      by forming dimers and tetramers. The purpose of this study was to evaluate the 
      relationship of telomere interactions to DNA damage, a factor known to adversely 
      influence male fertility. Telomeres were localized by fluorescence in situ 
      hybridization (FISH) using human chromosome pan-telomeric probe in ten samples 
      with low and ten samples with high sperm DNA damage. The samples with a low DNA 
      fragmentation index (DFI) had a mean number of telomere signals of 21.7+/-1.9 
      compared to a mean of 26.5+/-3.4 signals in the samples with a high DFI (p<.005). 
      The percentage of cells with a typical telomere distribution of </=23 
      telomere-telomere dimers was observed in 70.8%+/-15.6 samples with a low DFI 
      compared to 44.2%+/-22.4 in samples with a high DFI (p<.05). These results suggest 
      that sperm DNA damage is associated with disruption of the normal 
      telomere-telomere interactions leading to possible loss of the looped chromosome 
      configuration. Improperly packed and organized sperm chromatin might have a high 
      probability of disrupting the extremely structured sequence of sperm chromosome 
      deposition, activation, and processing by the oocyte at the time of 
      fertilization. These results might provide additional information on the nature 
      of sperm DNA damage and the role of such damage on fertilization and development 
      of the zygote.
FAU - Moskovtsev, Sergey I
AU  - Moskovtsev SI
AD  - Andrology Laboratory, Department of Pathology and Laboratory Medicine, Mount 
      Sinai Hospital, Toronto, Ontario, Canada. smoskovtsev@mtsinai.on.ca
FAU - Willis, Jennifer
AU  - Willis J
FAU - White, John
AU  - White J
FAU - Mullen, J Brendan M
AU  - Mullen JB
LA  - eng
PT  - Journal Article
DEP - 20101001
PL  - England
TA  - Syst Biol Reprod Med
JT  - Systems biology in reproductive medicine
JID - 101464963
RN  - 0 (Chromatin)
SB  - IM
MH  - Chromatin/ultrastructure
MH  - *DNA Fragmentation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Spermatozoa/*physiology
MH  - Telomere/*physiology/ultrastructure
EDAT- 2010/10/05 06:00
MHDA- 2011/03/01 06:00
CRDT- 2010/10/02 06:00
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/03/01 06:00 [medline]
AID - 10.3109/19396368.2010.502587 [doi]
PST - ppublish
SO  - Syst Biol Reprod Med. 2010 Dec;56(6):407-12. doi: 10.3109/19396368.2010.502587. 
      Epub 2010 Oct 1.