PMID- 20884369
OWN - NLM
STAT- MEDLINE
DCOM- 20110216
LR  - 20181201
IS  - 1089-8611 (Electronic)
IS  - 1089-8603 (Linking)
VI  - 23
IP  - 4
DP  - 2010 Dec 15
TI  - Expression of N-methyl-D-aspartate receptor and its effect on nitric oxide 
      production of rat alveolar macrophages.
PG  - 327-31
LID - 10.1016/j.niox.2010.09.004 [doi]
AB  - We early show that glutamate (Glu) mediate hyperoxia-induced newborn rat lung 
      injury through N-methyl-D-aspartate receptor (NMDAR). In this study, we search 
      for evidence of NMDAR expression on newborn rat alveolar macrophages (AMs) and 
      the difference between newborn and adult rat AMs, and the possible effect on 
      nitric oxide (NO) production of AMs by exogenous NMDA. The protein of NMDAR was 
      showed by immunocytochemistry, and the mRNA was examined by RT-PCR and real-time 
      PCR. The results show that: (i) both newborn and adult rat AMs express NMDAR1 and 
      the four NMDAR2 subtypes and newborn rat AMs are higher expression. (ii) NMDA 
      administration increase NO production, inducible nitric oxide synthase (iNOS) 
      activity and iNOS mRNA expression of AMs. (iii) NMDAR activation elevates NO 
      secretion of AMs, which suggests that AM may be one of the key cellular origin of 
      the elevated NO secretion in hyperoxia-induced lung injury.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Shang, L H
AU  - Shang LH
AD  - Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Luo, Z Q
AU  - Luo ZQ
FAU - Deng, X D
AU  - Deng XD
FAU - Wang, M J
AU  - Wang MJ
FAU - Huang, F R
AU  - Huang FR
FAU - Feng, D D
AU  - Feng DD
FAU - Yue, S J
AU  - Yue SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100925
PL  - United States
TA  - Nitric Oxide
JT  - Nitric oxide : biology and chemistry
JID - 9709307
RN  - 0 (NMDA receptor A1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - VH92ICR8HX (N-methyl D-aspartate receptor subtype 2A)
SB  - IM
MH  - Animals
MH  - Macrophages, Alveolar/*metabolism
MH  - N-Methylaspartate/administration & dosage
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/10/05 06:00
MHDA- 2011/02/17 06:00
CRDT- 2010/10/02 06:00
PHST- 2010/05/08 00:00 [received]
PHST- 2010/09/16 00:00 [revised]
PHST- 2010/09/17 00:00 [accepted]
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/02/17 06:00 [medline]
AID - S1089-8603(10)00418-0 [pii]
AID - 10.1016/j.niox.2010.09.004 [doi]
PST - ppublish
SO  - Nitric Oxide. 2010 Dec 15;23(4):327-31. doi: 10.1016/j.niox.2010.09.004. Epub 
      2010 Sep 25.