PMID- 20885848
OWN - NLM
STAT- MEDLINE
DCOM- 20110119
LR  - 20211020
IS  - 1928-9022 (Electronic)
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 74
IP  - 3
DP  - 2010 Jul
TI  - Serodiagnosis of porcine reproductive and respiratory syndrome virus infection 
      with the use of glycoprotein 5 antigens.
PG  - 223-7
AB  - Glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus 
      (PRRSV) has been studied extensively as a target for vaccine development. This 
      study evaluated the serodiagnostic application of PRRSV GP5 by enzyme-linked 
      immunosorbent assay (ELISA). Two immunodominant peptides (VR #1 and VR #2) and 
      two neutralizing ectodomain-containing peptides (Ecto #1 and Ecto #2), as well as 
      recombinant GP5 (rGP5) as a control, were prepared. Serum from unvaccinated pigs 
      was screened for the antibodies that bind to these peptide and protein antigens. 
      The results were compared with those from a commercially available diagnostic 
      ELISA kit (HerdChek), which uses the nucleocapsid (N) protein as an antigen. Only 
      VR #1+#2 showed a result statistically similar to that of N protein. Ecto #1 and 
      Ecto #2 had a lower sensitivity than VR #1+#2 and rGP5. The peptides and rGP5 
      showed significant associations with the N protein (P < 0.05 or 0.01), which 
      suggests that GP5 may also be a candidate serodiagnostic antigen. Since 
      antibodies against GP5 persist much longer than those against the N protein, GP5 
      itself and some of its fragments are thought to be good targets for 
      serodiagnosis. In addition, the presence of antibodies against the PRRSV 
      structural antigens showed significant antigen-dependent differences.
FAU - Pyo, Hyoju
AU  - Pyo H
AD  - Department of Veterinary Medicine, College of Veterinary Medicine, Chonnam 
      National University, Gwangju 500-757, Republic of Korea.
FAU - Seo, Jayoung
AU  - Seo J
FAU - Suh, Gukhyun
AU  - Suh G
FAU - Kim, Kwanghyun
AU  - Kim K
FAU - Lee, Jaeil
AU  - Lee J
FAU - Kim, Taejung
AU  - Kim T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Immunodominant Epitopes
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*physiology
MH  - Sensitivity and Specificity
MH  - Serologic Tests
MH  - Swine
MH  - Viral Envelope Proteins/*immunology
PMC - PMC2896805
EDAT- 2010/10/05 06:00
MHDA- 2011/01/20 06:00
PMCR- 2010/10/01
CRDT- 2010/10/02 06:00
PHST- 2009/02/25 00:00 [received]
PHST- 2009/09/30 00:00 [accepted]
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/01/20 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - cjvr_03_223 [pii]
PST - ppublish
SO  - Can J Vet Res. 2010 Jul;74(3):223-7.