PMID- 20886169
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20191210
IS  - 1464-0333 (Electronic)
IS  - 1464-0325 (Linking)
VI  - 12
IP  - 11
DP  - 2010 Nov
TI  - Optofluidic device monitoring and fluid dynamics simulation for the spread of 
      viral pathogens in a livestock environment.
PG  - 2138-44
LID - 10.1039/c0em00365d [doi]
AB  - Rapid monitoring of the spreads of porcine reproductive and respiratory syndrome 
      virus (PRRSV) was attempted using samples collected from nasal swabs of pigs and 
      air samplers within an experimental swine building. An optofluidic device 
      containing liquid-core waveguides was used to detect forward Mie light scattering 
      caused by the agglutination of anti-PRRSV-conjugated submicron particles, with 
      enhanced sensitivity, signal reproducibility, and reusability (reusable up to 75 
      assays). These results were compared with reverse transcription polymerase chain 
      reaction (RT-PCR) assays (35 cycles) and showed excellent agreements to them. 
      Each assay took less than 10 min including all necessary sample pre-processing, 
      while the RT-PCR assays took up to 4 h including sample pre-processing and gel 
      imaging for PCR products. A 3-D computational fluid dynamics (CFD) simulation was 
      utilized to track the transport and distribution of PRRSV (from the mouths of 
      pigs to the exhaust fans) within a swine building, and compared with the readings 
      from an optofluidic device. Simulation results corresponded well with the 
      experimental data, validating our 3-D CFD model for the spread of viral pathogens 
      in a livestock environment. The developed optofluidic device and 3-D CFD model 
      can serve as a good model for monitoring the spread of influenza A (swine and 
      avian) within animal and human environments.
FAU - Kwon, Hyuck-Jin
AU  - Kwon HJ
AD  - Department of Agricultural & Biosystems Engineering, The University of Arizona, 
      Tucson, Arizona 85721-0038, United States.
FAU - Lee, Chang-Hee
AU  - Lee CH
FAU - Choi, Eun-Jin
AU  - Choi EJ
FAU - Song, Jae-Young
AU  - Song JY
FAU - Heinze, Brian C
AU  - Heinze BC
FAU - Yoon, Jeong-Yeol
AU  - Yoon JY
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101001
PL  - England
TA  - J Environ Monit
JT  - Journal of environmental monitoring : JEM
JID - 100968688
RN  - 0 (Antibodies)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Environmental Monitoring/*instrumentation
MH  - Equipment Design
MH  - Immunoassay/instrumentation
MH  - Light
MH  - *Optical Devices
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Scattering, Radiation
MH  - Sus scrofa/*virology
EDAT- 2010/10/05 06:00
MHDA- 2011/03/11 06:00
CRDT- 2010/10/02 06:00
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
AID - 10.1039/c0em00365d [doi]
PST - ppublish
SO  - J Environ Monit. 2010 Nov;12(11):2138-44. doi: 10.1039/c0em00365d. Epub 2010 Oct 
      1.