PMID- 20886204
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20211020
IS  - 1432-0428 (Electronic)
IS  - 0012-186X (Print)
IS  - 0012-186X (Linking)
VI  - 53
IP  - 12
DP  - 2010 Dec
TI  - mRNA expression analysis of cell cycle genes in islets of pregnant mice.
PG  - 2579-88
LID - 10.1007/s00125-010-1912-8 [doi]
AB  - AIMS/HYPOTHESIS: Pregnancy requires an increase in the functional beta cell mass 
      to match metabolic needs for insulin. To understand this adaptation at the 
      molecular level, we undertook a time course analysis of mRNA expression in mice. 
      METHODS: Total RNA extracted from C57Bl6/J mouse islets every 3 days during 
      pregnancy was hybridised on commercially available expression arrays. Gene 
      network analysis was performed and changes in functional clusters over time 
      visualised. The function of putative novel cell cycle genes was assessed via 
      silencing in replicating mouse insulinoma 6 (MIN6) cells. RESULTS: Gene network 
      analysis identified a large gene cluster associated with cell cycle control (67 
      genes, all upregulated by >/= 1.5-fold, p < 0.001). The number of upregulated cell 
      cycle genes and the mRNA expression levels of individual genes peaked at 
      pregnancy day (P)9.5. Filtering of poorly annotated genes with enhanced 
      expression in islets at P9.5, and in MIN6 cells and thymus resulted in further 
      studies with G7e (also known as D17H6S56E-5) and Fignl1. Gene knock-down 
      experiments in MIN6 cells suggested that these genes are indeed involved in 
      adequate cell cycle accomplishment. CONCLUSIONS/INTERPRETATION: A sharp peak of 
      cell cycle-related mRNA expression in islets occurs around P9.5, after which beta 
      cell replication is increased. As illustrated by the identification of G7e and 
      Fignl1 in islets of pregnant mice, further study of this distinct transcriptional 
      peak should help to unravel the complex process of beta cell replication.
FAU - Schraenen, A
AU  - Schraenen A
AD  - Gene Expression Unit, Department of Molecular Cell Biology, Katholieke 
      Universiteit Leuven, Herestraat 49, mailbox 901, 3000 Leuven, Belgium.
FAU - de Faudeur, G
AU  - de Faudeur G
FAU - Thorrez, L
AU  - Thorrez L
FAU - Lemaire, K
AU  - Lemaire K
FAU - Van Wichelen, G
AU  - Van Wichelen G
FAU - Granvik, M
AU  - Granvik M
FAU - Van Lommel, L
AU  - Van Lommel L
FAU - in't Veld, P
AU  - in't Veld P
FAU - Schuit, F
AU  - Schuit F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101001
PL  - Germany
TA  - Diabetologia
JT  - Diabetologia
JID - 0006777
RN  - 0 (D17H6S56E-5 protein, mouse)
RN  - 0 (HLA Antigens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Knockdown Techniques
MH  - *Genes, cdc
MH  - HLA Antigens/genetics/metabolism
MH  - Insulin-Secreting Cells/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pregnancy
MH  - RNA, Messenger/analysis/*genetics/metabolism
MH  - Time Factors
PMC - PMC2974927
EDAT- 2010/10/05 06:00
MHDA- 2011/02/25 06:00
PMCR- 2010/10/01
CRDT- 2010/10/02 06:00
PHST- 2010/05/19 00:00 [received]
PHST- 2010/08/17 00:00 [accepted]
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - 1912 [pii]
AID - 10.1007/s00125-010-1912-8 [doi]
PST - ppublish
SO  - Diabetologia. 2010 Dec;53(12):2579-88. doi: 10.1007/s00125-010-1912-8. Epub 2010 
      Oct 1.