PMID- 20886881
OWN - NLM
STAT- MEDLINE
DCOM- 20141125
LR  - 20140204
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 58
IP  - 20
DP  - 2010 Oct 27
TI  - Egg-derived peptide IRW inhibits TNF-alpha-induced inflammatory response and 
      oxidative stress in endothelial cells.
PG  - 10840-6
LID - 10.1021/jf102120c [doi]
AB  - Oxidative stress and vascular inflammatory response are key mediators of 
      endothelial dysfunction that leads to cardiovascular diseases. A novel peptide, 
      IRW, was previously characterized from egg protein with angiotensin converting 
      enzyme inhibitory activity. The purpose of the study was to investigate the 
      effects and molecular mechanisms of IRW on regulating inflammatory response in 
      endothelial cells. The results showed that tumor necrosis factor-alpha (TNF-alpha) 
      significantly increased the protein levels of intercellular adhesion molecule-1 
      (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and monocyte chemotactic 
      protein-1 (MCP-1), whereas pretreatment with IRW inhibited TNF-alpha-induced 
      increases of ICAM-1, VCAM-1, and MCP-1 production in a concentration-dependent 
      manner. IRW also reduced the levels of superoxide ions in the presence and 
      absence of TNF-alpha. These results indicate the potential role of IRW in preventing 
      cardiovascular disease as a functional food ingredient or nutraceutical.
FAU - Huang, Wuyang
AU  - Huang W
AD  - Department of Agricultural, Food and Nutritional Science, Faculty of 
      Agricultural, Life and Environmental Sciences, University of Alberta, Edmonton, 
      Alberta, Canada.
FAU - Chakrabarti, Subhadeep
AU  - Chakrabarti S
FAU - Majumder, Kaustav
AU  - Majumder K
FAU - Jiang, Yanyan
AU  - Jiang Y
FAU - Davidge, Sandra T
AU  - Davidge ST
FAU - Wu, Jianping
AU  - Wu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101001
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Egg Proteins)
RN  - 0 (Peptides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/genetics/immunology
MH  - Chickens
MH  - Egg Proteins/*chemistry
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/immunology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/immunology
MH  - Oxidative Stress/*drug effects
MH  - Peptides/*pharmacology
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology
MH  - Vascular Cell Adhesion Molecule-1/genetics/immunology
EDAT- 2010/10/05 06:00
MHDA- 2014/12/15 06:00
CRDT- 2010/10/05 06:00
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1021/jf102120c [doi]
PST - ppublish
SO  - J Agric Food Chem. 2010 Oct 27;58(20):10840-6. doi: 10.1021/jf102120c. Epub 2010 
      Oct 1.