PMID- 20887549
OWN - NLM
STAT- MEDLINE
DCOM- 20101027
LR  - 20211020
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 43
IP  - 5
DP  - 2010 Oct
TI  - Differentiation of bone marrow-derived mesenchymal stem cells into 
      hepatocyte-like cells in an alginate scaffold.
PG  - 427-34
LID - 10.1111/j.1365-2184.2010.00692.x [doi]
AB  - OBJECTIVES: Alginate scaffolds are the most frequently investigated biomaterials 
      in tissue engineering. Tissue engineering techniques that generate liver tissue 
      have become important for treatment of a number of liver diseases and recent 
      studies indicate that bone marrow-derived stem cells (BMSCs) can differentiate 
      into hepatocyte-like cells. The goal of the study described here, was to examine 
      in vitro hepatic differentiation potential of BMSCs cultured in an alginate 
      scaffold. MATERIALS AND METHODS: To investigate the potential of BMSCs to 
      differentiate into hepatocyte-like cells, we cultured BMSCs in alginate scaffolds 
      in the presence of specific growth factors including hepatocyte growth factor, 
      epidermal growth factor and fibroblast growth factor-4. RESULTS: We can 
      demonstrate that alginate scaffolds are compatible for growth of BMSCs and when 
      cultured in alginate scaffolds for several days they display several 
      liver-specific markers and functions. Specifically, they expressed genes encoding 
      alpha-foetoprotein, albumin (ALB), connexin 32 and CYP7A1. In addition, these 
      BMSCs produced both ALB and urea, expressed cytokeratin-18 (CK-18) and were 
      capable of glycogen storage. Percentage of CK-18 positive cells, a marker of 
      hepatocytes, was 56.7%. CONCLUSIONS: Our three-dimensional alginate scaffolds 
      were highly biocompatible with BMSCs. Furthermore, culturing induced their 
      differentiation into hepatocyte-like cells. Therefore, BMSCs cultured in alginate 
      scaffolds may be applicable for hepatic tissue engineering.
FAU - Lin, N
AU  - Lin N
AD  - Department of Hepatobiliary Surgery, the Third Affiliated Hospital, Sun Yat-Sen 
      University, GuangZhou, China.
FAU - Lin, J
AU  - Lin J
FAU - Bo, L
AU  - Bo L
FAU - Weidong, P
AU  - Weidong P
FAU - Chen, S
AU  - Chen S
FAU - Xu, R
AU  - Xu R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Albumins)
RN  - 0 (Alginates)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Fibroblast Growth Factor 4)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Keratin-18)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 8W8T17847W (Urea)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Albumins/metabolism
MH  - *Alginates/metabolism
MH  - Animals
MH  - Biocompatible Materials
MH  - Bone Marrow Cells/*cytology
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/administration & dosage
MH  - Fibroblast Growth Factor 4/administration & dosage
MH  - Glucuronic Acid/metabolism
MH  - Glycogen/metabolism
MH  - Hepatocyte Growth Factor/administration & dosage
MH  - Hepatocytes/*cytology
MH  - Hexuronic Acids/metabolism
MH  - Keratin-18/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tissue Scaffolds
MH  - Urea/metabolism
PMC - PMC6496777
EDAT- 2010/10/05 06:00
MHDA- 2010/10/28 06:00
PMCR- 2010/08/30
CRDT- 2010/10/05 06:00
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2010/10/28 06:00 [medline]
PHST- 2010/08/30 00:00 [pmc-release]
AID - CPR692 [pii]
AID - 10.1111/j.1365-2184.2010.00692.x [doi]
PST - ppublish
SO  - Cell Prolif. 2010 Oct;43(5):427-34. doi: 10.1111/j.1365-2184.2010.00692.x.