PMID- 20889010
OWN - NLM
STAT- MEDLINE
DCOM- 20110204
LR  - 20101004
IS  - 0399-8320 (Print)
IS  - 0399-8320 (Linking)
VI  - 34 Suppl 1
DP  - 2010 Sep
TI  - [Functional biostructure of colonic microbiota (central fermenting area, germinal 
      stock area and separating mucus layer) in healthy subjects and patients with 
      diarrhea treated with Saccharomyces boulardii].
PG  - S79-92
LID - 10.1016/S0399-8320(10)70025-7 [doi]
AB  - The colonic content can be compared to a spatially structured high output 
      bioreactor composed of three functionally different regions: a separating mucus 
      layer, a germinal stock area, and a central fermenting area. The stool mirrors 
      this structure and can be used for diagnosis in health and disease. In a first 
      part, we introduce a novel method based on fluorescence in situ hybridization 
      (FISH) of sections of punched-out stool cylinders, which allows quantitatively 
      monitor microbiota in the mucus, the germinal stock and the central fermenting 
      areas. in a second part, we demonstrate the practical implementation of this 
      method, describing the biostructure of stool microbiota in healthy subjects and 
      patients with chronic idiopathic diarrhea treated with Saccharomyces boulardii. 
      Punched stool cylinders from 20 patients with chronic idiopathic diarrhea and 20 
      healthy controls were investigated using fluorescence in situ hybridization. 
      Seventy-three bacterial groups were evaluated. Fluctuations in assembly of 11 
      constitutive bacterial groups were monitored weekly for 3 weeks prior to, 3 weeks 
      during, and 3 weeks after oral Saccharomyces boulardii supplementation. Typical 
      findings in healthy subjects were a 5-60 mum mucus separating layer; homogeneous 
      distribution and fluorescence, high concentrations (>10 x 10(10) bacterial/mL) of 
      the three habitual bacterial groups: Bacteroides, Roseburia and Faecalibacterium 
      prausnitzii; and low concentrations of the occasional bacterial groups. The 
      diarrhea could be described in terms of increased separating effort, purging, 
      decontamination, bacterial substitution. Typical findings in diarrhea were: 
      increased thickness of the protective mucus layer, its incorporation in the 
      stool, absolute reduction in concentrations of the habitual bacterial groups, 
      suppression of bacterial metabolism in the central fermenting area (hybridization 
      silence), stratification of the stool structure by watery ingredients, and 
      substitutive increase in the concentrations of occasional bacterial groups. The 
      microbial and clinical symptoms of diarrhea were reversible with Saccharomyces 
      boulardii therapy. The structure-functional analysis of stool microbiota allows 
      to quantitatively monitor colonic malfunction and its response to therapy. 
      Saccharomyces boulardii significantly improves the stool biostructure in patients 
      with chronic idiopathic diarrhea and has no influence on the stool microbiota in 
      healthy subjects.
CI  - Copyright (c) 2010 Elsevier Masson SAS. All rights reserved.
FAU - Swidsinski, A
AU  - Swidsinski A
AD  - Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial 
      Biofilms and Department of Medicine, Section of Gastroenterology, Charite 
      Universitatsmedizin Berlin, Berlin, Germany. alexander.swidsinski@charite.de
FAU - Loening-Baucke, V
AU  - Loening-Baucke V
FAU - Kirsch, S
AU  - Kirsch S
FAU - Doerffel, Y
AU  - Doerffel Y
LA  - fre
PT  - English Abstract
PT  - Journal Article
TT  - Biostructure fonctionnelle du microbiote colique(zone de fermentation centrale, 
      zone de reservegerminale et couche de mucus separatrice) chez lessujets sains et 
      chez les patients atteints de diarrheetraites par Saccharomyces boulardii.
PL  - France
TA  - Gastroenterol Clin Biol
JT  - Gastroenterologie clinique et biologique
JID - 7704825
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Chronic Disease
MH  - Colon/*microbiology
MH  - Diarrhea/metabolism/*microbiology/therapy
MH  - Feces/chemistry/*microbiology
MH  - Female
MH  - Fermentation/drug effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Metagenome
MH  - Middle Aged
MH  - Mucus/drug effects/metabolism
MH  - Probiotics/administration & dosage/*therapeutic use
MH  - *Saccharomyces
MH  - Time Factors
MH  - Treatment Outcome
EDAT- 2010/10/05 06:00
MHDA- 2011/02/05 06:00
CRDT- 2010/10/05 06:00
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/02/05 06:00 [medline]
AID - S0399-8320(10)70025-7 [pii]
AID - 10.1016/S0399-8320(10)70025-7 [doi]
PST - ppublish
SO  - Gastroenterol Clin Biol. 2010 Sep;34 Suppl 1:S79-92. doi: 
      10.1016/S0399-8320(10)70025-7.