PMID- 20889231
OWN - NLM
STAT- MEDLINE
DCOM- 20110420
LR  - 20240109
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 168
IP  - 3
DP  - 2011 Feb 15
TI  - Ascorbate-glutathione cycle of mitochondria in osmoprimed soybean cotyledons in 
      response to imbibitional chilling injury.
PG  - 226-32
LID - 10.1016/j.jplph.2010.07.017 [doi]
AB  - Osmopriming treatment of chilling-sensitive soybean (Glycine max L. cv. 
      Zhonghuang-22) seeds for 72h at 25 degrees C with polyethylene glycol (PEG8000) solution 
      at -1.5MPa strongly improves chilling resistance. The aim of the present work was 
      to investigate whether the beneficial effect of osmopriming is associated with 
      restoration of the ascorbate-glutathione (ASC-GSH) cycle of mitochondria in 
      soybean seeds. Compared with the control, both H(2)O(2) and malondialdehyde (MDA) 
      contents in mitochondria of osmoprimed seeds decreased after chilling treatment, 
      and these changes were associated with increased activities of superoxide 
      dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), 
      monodehydroascorbate reductase (MDHAR) and L-galactono-gamma-lactone dehydrogenase 
      (GLDH). However, the activity of dehydroascorbate reductase (DHAR) showed no 
      obvious change during osmopriming treatment. Increased ASC and GSH contents 
      accompanied prolonged osmopriming, and the reduced/oxidized ratios of ASC and GSH 
      increased differently during osmopriming. These results indicate that osmopriming 
      treatment enhances activity of the ASC-GSH cycle of mitochondria, which raises 
      the chilling tolerance in soybean seeds and protects against H(2)O(2) that is 
      generated in mitochondria during imbibition at low temperature.
CI  - Copyright A(c) 2010 Elsevier GmbH. All rights reserved.
FAU - Sun, Haiping
AU  - Sun H
AD  - Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, 
      Ministry of Education, Beijing Forestry University, Beijing 100083, China.
FAU - Li, Lin
AU  - Li L
FAU - Wang, Xu
AU  - Wang X
FAU - Wu, Sen
AU  - Wu S
FAU - Wang, Xiaofeng
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - GAN16C9B8O (Glutathione)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Ascorbic Acid/*metabolism
MH  - Cold Temperature
MH  - Cotyledon/*metabolism
MH  - Germination/physiology
MH  - Glutathione/*metabolism
MH  - Hydrogen Peroxide/metabolism
MH  - Lipid Peroxidation
MH  - Mitochondria/*metabolism
MH  - Glycine max/*metabolism
EDAT- 2010/10/05 06:00
MHDA- 2011/04/22 06:00
CRDT- 2010/10/05 06:00
PHST- 2009/11/13 00:00 [received]
PHST- 2010/07/06 00:00 [revised]
PHST- 2010/07/07 00:00 [accepted]
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - S0176-1617(10)00399-8 [pii]
AID - 10.1016/j.jplph.2010.07.017 [doi]
PST - ppublish
SO  - J Plant Physiol. 2011 Feb 15;168(3):226-32. doi: 10.1016/j.jplph.2010.07.017.