PMID- 20890458
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20211203
IS  - 2041-4889 (Electronic)
VI  - 1
IP  - 1
DP  - 2010
TI  - Activation of mTOR coincides with autophagy during ligation-induced atrophy in 
      the rat submandibular gland.
PG  - e14
LID - 10.1038/cddis.2009.12 [doi]
AB  - Salivary gland atrophy is a common consequence of pathology, including Sjogren's 
      syndrome, irradiation therapy and obstructive sialadenitis. During severe atrophy 
      of the rat submandibular gland caused by excretory duct ligation, the majority of 
      acinar cells disappear through apoptosis, whereas ductal cells proliferate and 
      dedifferentiate; yet, the gland can survive in the atrophic state almost 
      indefinitely, with an ability to fully recover if deligated. The control 
      mechanisms governing these observations are not well understood. We report that 
      ~10% of acinar cells survive in ligation-induced atrophy. Microarray and 
      quantitative real-time PCR analysis of ligated glands indicated sustained 
      transcription of acinar cell-specific genes, whereas ductal-specific genes were 
      reduced to background levels. After 3 days of ligation, activation of the 
      mammalian target of rapamycin (mTOR) pathway and autophagy occurred as shown by 
      phosphorylation of 4E-BP1 and expression of autophagy-related proteins. These 
      results suggest that activation of mTOR and the autophagosomal pathway are 
      important mechanisms that may help to preserve acinar cells during atrophy of 
      salivary glands after injury.
FAU - Silver, N
AU  - Silver N
AD  - King's College London, Dental Institute, London, UK.
FAU - Proctor, G B
AU  - Proctor GB
FAU - Arno, M
AU  - Arno M
FAU - Carpenter, G H
AU  - Carpenter GH
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - 079066/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (Aqp5 protein, rat)
RN  - 0 (Aquaporin 5)
RN  - 0 (Carrier Proteins)
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LOC100363344)
RN  - 0 (Phosphoproteins)
RN  - 0 (Salivary Proteins and Peptides)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Aquaporin 5/metabolism
MH  - Atrophy
MH  - *Autophagy
MH  - Carrier Proteins/metabolism
MH  - Gene Expression Regulation
MH  - Intracellular Signaling Peptides and Proteins
MH  - Ligation
MH  - Male
MH  - Phosphoproteins/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Salivary Proteins and Peptides/metabolism
MH  - Signal Transduction
MH  - Submandibular Gland/cytology/*metabolism/*pathology
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC2948542
MID - UKMS30070
OTO - NOTNLM
OT  - atrophy
OT  - autophagy
OT  - mTOR
OT  - submandibular gland
EDAT- 2010/10/05 06:00
MHDA- 2011/06/24 06:00
PMCR- 2010/01/01
CRDT- 2010/10/05 06:00
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
PHST- 2010/01/01 00:00 [pmc-release]
AID - cddis200912 [pii]
AID - 10.1038/cddis.2009.12 [doi]
PST - ppublish
SO  - Cell Death Dis. 2010;1(1):e14. doi: 10.1038/cddis.2009.12.