PMID- 2091667
OWN - NLM
STAT- MEDLINE
DCOM- 19910607
LR  - 20191029
IS  - 1044-7393 (Print)
IS  - 1044-7393 (Linking)
VI  - 12
IP  - 3
DP  - 1990 Jun
TI  - Biochemical changes in central nervous system membranes in chronic-relapsing 
      experimental allergic encephalomyelitis.
PG  - 229-41
AB  - Biochemical studies of myelin fractions were undertaken on Lewis rats during 
      various time-points in the development of chronic-relapsing experimental allergic 
      encephalomyelitis (CR-EAE). Lipid and protein composition of myelin fractions 
      obtained by sucrose density gradient centrifugation at 10, 19, 24, and 66 d 
      postinduction (pi) were determined by high-performance thin-layer chromatography 
      (HPTLC) and sodium dodecyl sulfate-polyacrilamide gel electrophoresis (SDS PAGE), 
      respectively. When comparing the myelin fractions of CR-EAE affected animals with 
      those of controls, main differences were observed at 10 d pi. These changes were 
      particularly evident in the light myelin fraction, where a decrease in the 
      percentage of phosphatidylethanolamine and small basic protein relative to the 
      total lipids and proteins of the fraction were observed. At 19 and 24 d pi no 
      biochemical differences were present in both fractions. At 66 d pi, differences 
      in the lipid composition were observed again only in the light myelin fraction. 
      These findings suggest that the light myelin fraction is the most sensitive, 
      particularly at the early stages of the disease, and must play a key role in 
      demyelinating processes.
FAU - Salvati, S
AU  - Salvati S
AD  - Istituto Superiore di Sanita, Rome, Italy.
FAU - Confaloni, A
AU  - Confaloni A
FAU - DiBiase, A
AU  - DiBiase A
FAU - Attorri, L
AU  - Attorri L
FAU - Serlupi Crescenzi, G
AU  - Serlupi Crescenzi G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Chem Neuropathol
JT  - Molecular and chemical neuropathology
JID - 8910358
RN  - 0 (Lipids)
RN  - 0 (Myelin Proteins)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Centrifugation, Density Gradient
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Thin Layer
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Encephalomyelitis, Autoimmune, Experimental/metabolism/*physiopathology
MH  - Lipids/isolation & purification
MH  - Male
MH  - Myelin Proteins/isolation & purification/*metabolism
MH  - Myelin Sheath/*metabolism/ultrastructure
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Reference Values
MH  - Time Factors
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - 10.1007/BF03159947 [doi]
PST - ppublish
SO  - Mol Chem Neuropathol. 1990 Jun;12(3):229-41. doi: 10.1007/BF03159947.