PMID- 20921293
OWN - NLM
STAT- MEDLINE
DCOM- 20110927
LR  - 20151119
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 26
IP  - 5
DP  - 2011 May
TI  - Matrix metalloproteinase levels in the drained dialysate reflect the peritoneal 
      solute transport rate: a multicentre study in Japan.
PG  - 1695-701
LID - 10.1093/ndt/gfq593 [doi]
AB  - BACKGROUND: Long-term peritoneal dialysis (PD) leads to peritoneal injury with 
      high solute transport of the peritoneal membrane. At worst, peritoneal injury 
      leads to encapsulating peritoneal sclerosis with an extremely high mortality 
      rate. To perform PD safely and adequately, it is necessary to monitor peritoneal 
      injury. The aim of this study was to investigate the potential of matrix 
      metalloproteinases (MMPs) as new indicators of peritoneal injury. METHODS: The 
      subjects included 215 PD patients with end-stage renal disease at 20 centres in 
      Japan. MMPs or tissue inhibitors of MMP (TIMPs) in the drained dialysate were 
      quantified with enzyme-linked immunosorbent assay. The peritoneal solute 
      transport rate was assessed to estimate peritoneal injury and PD efficiency by 
      the peritoneal equilibration test (PET). RESULTS: MMP-2, MMP-3 and TIMP-1 levels 
      in the drained dialysate obtained by the PET were correlated with the D/P Cr 
      ratios (rho = 0.69, rho = 0.52, rho = 0.55, respectively) and the D/D0 glucose ratios 
      (rho = -0.60, rho = -0.47, rho = -0.48, respectively). The measured D/S ratios of MMP-2 
      and TIMP-1 were significantly higher than the expected D/S ratios when MMP-2 and 
      TIMP-1 would have been transported from only the circulation. The measured D/S 
      ratios of MMP-3 nearly corresponded to the expected ratios. MMP-1 and TIMP-2 in 
      the drainage were undetected in most patients. CONCLUSIONS: From these results, 
      most MMP-2 in the drained dialysate may be produced from the peritoneum, and 
      MMP-2 is expected to be a useful marker of peritoneal injury or change in 
      peritoneal solute transport.
FAU - Hirahara, Ichiro
AU  - Hirahara I
AD  - Division of Nephrology, Department of Internal Medicine, Jichi Medical 
      University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. 
      hirahara@rpf.jp.
FAU - Inoue, Makoto
AU  - Inoue M
FAU - Umino, Tetsuo
AU  - Umino T
FAU - Saito, Osamu
AU  - Saito O
FAU - Muto, Shigeaki
AU  - Muto S
FAU - Kusano, Eiji
AU  - Kusano E
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20101004
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Biomarkers)
RN  - 0 (Dialysis Solutions)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Dialysis Solutions/*pharmacokinetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Japan
MH  - Kidney Failure, Chronic/enzymology/*therapy
MH  - Male
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Middle Aged
MH  - Peritoneal Dialysis/*adverse effects
MH  - Peritoneum/*enzymology/*injuries/pathology
MH  - Prognosis
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
EDAT- 2010/10/06 06:00
MHDA- 2011/09/29 06:00
CRDT- 2010/10/06 06:00
PHST- 2010/10/06 06:00 [entrez]
PHST- 2010/10/06 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - gfq593 [pii]
AID - 10.1093/ndt/gfq593 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2011 May;26(5):1695-701. doi: 10.1093/ndt/gfq593. Epub 
      2010 Oct 4.