PMID- 20929721
OWN - NLM
STAT- MEDLINE
DCOM- 20101223
LR  - 20211020
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 89
IP  - 12
DP  - 2010 Dec
TI  - Stem cell-calcium phosphate constructs for bone engineering.
PG  - 1482-8
LID - 10.1177/0022034510384623 [doi]
AB  - While human bone-marrow-derived mesenchymal stem cells (hBMSCs) have been 
      investigated, human umbilical cord mesenchymal stem cells (hUCMSCs) are a 
      relatively new cell source. Little has been reported on hUCMSC encapsulation in 
      scaffolds for bone tissue engineering. The objective of this study was to 
      encapsulate hBMSCs and hUCMSCs in calcium phosphate cement (CPC) scaffolds for 
      dental, craniofacial, and orthopedic applications. Stem-cell-encapsulating CPC 
      construct with chitosan and fiber reinforcement reached the strength of 
      cancellous bone, which was much stronger than previous injectable carriers for 
      cell delivery. hUCMSCs and hBMSCs inside the constructs showed excellent 
      viability and osteo-differentiation. The encapsulated hUCMSCs synthesized nearly 
      three-fold more bone minerals than the hBMSCs in vitro. Hence, 
      stem-cell-encapsulating CPC-chitosan-fiber construct may be promising for dental 
      and orthopedic applications. This study indicated that the hUCMSCs were a potent 
      alternative to the gold-standard hBMSCs, which may have a broad impact on 
      regenerative medicine and dental tissue engineering.
FAU - Xu, H H K
AU  - Xu HH
AD  - Department of Endodontics, Prosthodontics and Operative Dentistry, University of 
      Maryland Dental School, 650 West Baltimore Street, Baltimore, MD 21201, USA. 
      hxu@umaryland.edu
FAU - Zhao, L
AU  - Zhao L
FAU - Weir, M D
AU  - Weir MD
LA  - eng
GR  - R01 DE014190-09/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-07A1S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE017974/DE/NIDCR NIH HHS/United States
GR  - R01DE14190/DE/NIDCR NIH HHS/United States
GR  - R01DE17974/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-08/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101006
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
RN  - 0 (tetracalcium phosphate)
RN  - 104982-03-8 (Osteocalcin)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 34346-01-5 (Polyglactin 910)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - L11K75P92J (calcium phosphate, dibasic, anhydrous)
SB  - IM
MH  - Alkaline Phosphatase/analysis
MH  - Biocompatible Materials/chemistry
MH  - Biomechanical Phenomena
MH  - Bone Cements/*chemistry
MH  - Bone Density/physiology
MH  - Bone Marrow Cells/cytology
MH  - Bone Regeneration/physiology
MH  - *Bone and Bones/physiology
MH  - Calcium Phosphates/*chemistry
MH  - Cell Adhesion/physiology
MH  - Cell Differentiation/physiology
MH  - Cell Survival/physiology
MH  - Chitosan/chemistry
MH  - Elastic Modulus
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry
MH  - Mesenchymal Stem Cells/*physiology
MH  - Microscopy, Electron, Scanning
MH  - Osteocalcin/analysis
MH  - Pliability
MH  - Polyglactin 910/chemistry
MH  - Stress, Mechanical
MH  - Surface Properties
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
PMC - PMC3037524
MID - NIHMS267841
EDAT- 2010/10/12 06:00
MHDA- 2010/12/25 06:00
PMCR- 2011/12/01
CRDT- 2010/10/09 06:00
PHST- 2010/10/09 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2010/12/25 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 0022034510384623 [pii]
AID - 10.1177_0022034510384623 [pii]
AID - 10.1177/0022034510384623 [doi]
PST - ppublish
SO  - J Dent Res. 2010 Dec;89(12):1482-8. doi: 10.1177/0022034510384623. Epub 2010 Oct 
      6.