PMID- 20930032
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20101108
IS  - 1758-1001 (Electronic)
IS  - 0004-5632 (Linking)
VI  - 47
IP  - Pt 6
DP  - 2010 Nov
TI  - Effect of recipient-derived cells on the progression of familial amyloidotic 
      polyneuropathy after liver transplantation: a retrospective study.
PG  - 529-34
LID - 10.1258/acb.2010.010156 [doi]
AB  - BACKGROUND: Some familial amyloidotic polyneuropathy (FAP) patients show the 
      post-transplant progression of the clinical symptoms. Although the presence of 
      recipient-derived cells in transplanted livers has been reported, no studies 
      investigating the functional significance of this post-transplant chimerism in 
      transplanted FAP patients were performed. The aims of this study were to evaluate 
      amyloidogenic transthyretin (ATTR) production of recipient-derived cells and the 
      relationship between the protein from recipient-derived cells and the progression 
      of FAP symptoms after liver transplantation (LT). METHODS: Seven FAP ATTR 
      Val30Met patients who underwent LT were included in this study. In one male 
      patient with sex-mismatched donor, fluorescence in situ hybridization (FISH) 
      method was performed on a liver biopsy sample using DNA probes for visualizing X 
      and Y chromosomes to detect the recipient-derived cells. In three patients 
      including the FISH-analysed patient, ATTR mRNA expression in transplanted livers 
      was evaluated by the polymerase chain reaction (PCR)-restriction fragment length 
      polymorphism method and realtime quantitative reverse transcription-PCR. In five 
      of the seven patients, ATTR in serum protein expression was measured by mass 
      spectrometry. RESULTS: One FAP patient has 3.1% recipient-derived cells in the 
      transplanted liver. The ATTR mRNA was not expressed in any of the three 
      transplanted livers. The ATTR was not detected in any sera of the sampled 
      patients. CONCLUSION: Although the FAP patient had recipient-derived cells in the 
      transplanted liver, the recipient-derived cells did not contribute to the 
      production of ATTR in our specific case. The effect of recipient-derived cells on 
      the post-transplant progression of FAP symptoms may be negligible.
FAU - Ohya, Yuki
AU  - Ohya Y
AD  - Department of Transplantation and Pediatric Surgery, Graduate School of Medical 
      Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, Japan.
FAU - Jono, Hirofumi
AU  - Jono H
FAU - Nakamura, Masaaki
AU  - Nakamura M
FAU - Hayashida, Shintaro
AU  - Hayashida S
FAU - Ueda, Mitsuharu
AU  - Ueda M
FAU - Obayashi, Konen
AU  - Obayashi K
FAU - Misumi, Shogo
AU  - Misumi S
FAU - Asonuma, Katsuhiro
AU  - Asonuma K
FAU - Ando, Yukio
AU  - Ando Y
FAU - Inomata, Yukihiro
AU  - Inomata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101007
PL  - England
TA  - Ann Clin Biochem
JT  - Annals of clinical biochemistry
JID - 0324055
RN  - 0 (Amyloidogenic Proteins)
RN  - 0 (Prealbumin)
SB  - IM
MH  - Adult
MH  - Amyloid Neuropathies, Familial/*genetics/metabolism/*therapy
MH  - Amyloidogenic Proteins/genetics
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver/cytology/metabolism
MH  - *Liver Transplantation/adverse effects
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Prealbumin/genetics
MH  - Retrospective Studies
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2010/10/12 06:00
MHDA- 2011/02/23 06:00
CRDT- 2010/10/09 06:00
PHST- 2010/10/09 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
AID - acb.2010.010156 [pii]
AID - 10.1258/acb.2010.010156 [doi]
PST - ppublish
SO  - Ann Clin Biochem. 2010 Nov;47(Pt 6):529-34. doi: 10.1258/acb.2010.010156. Epub 
      2010 Oct 7.