PMID- 20930398
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20190706
IS  - 1347-5223 (Electronic)
IS  - 0009-2363 (Linking)
VI  - 58
IP  - 10
DP  - 2010 Oct
TI  - Synthesis, cytotoxicity and pro-apoptosis of novel benzoisoindolin hydrazones as 
      anticancer agents.
PG  - 1324-7
AB  - A series of benzoisoindolin hydrazones as analogues of natural lignan diphyllin 
      were synthesized and the structures of these compounds were established by 
      (1)H-NMR, (13)C-NMR, Mass and high resolution (HR)-MS. The compounds were 
      evaluated for in vitro cytotoxicity against KB, A549 and HCT-116 cancer cell 
      lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 
      assay. Compound 4 possessed the highest growth inhibitory effect. Significant 
      apoptosis of HCT-116 cells treated with compound 4 was observed by 
      Hoechst33342-propidium iodide (PI) and acridine orange (AO)-ethidium bromide (EB) 
      staining assay. Western blot analysis disclosed that compound 4 induced apoptosis 
      via the mitochondrial pathway accompanied by an increased expression of Bax and a 
      decreased expression of Bcl-2.
FAU - Zhao, Yu
AU  - Zhao Y
AD  - Institute of Nautical Medicine, Nantong University, People's Republic of China.
FAU - Hui, Jie
AU  - Hui J
FAU - Wang, Dan
AU  - Wang D
FAU - Zhu, Li
AU  - Zhu L
FAU - Fang, Jing-Huai
AU  - Fang JH
FAU - Zhao, Xiao-Dong
AU  - Zhao XD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Hydrazones)
RN  - 0 (Indoles)
RN  - 0 (Isoindoles)
RN  - 0 (N-(4-methylbenzylidenyl)-4'-(3'',4''-methylenedioxyphenyl)-6',7'- 
      dimethoxyl-1'-hydroxyl-benzyl-2,3-dihydro-isoindol-1-hydrazone)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 6DPT9AB2NK (indoline)
SB  - IM
MH  - Antineoplastic Agents/*chemical synthesis/therapeutic use/toxicity
MH  - *Apoptosis
MH  - Cell Line, Tumor
MH  - Humans
MH  - Hydrazones/chemical synthesis/*chemistry/therapeutic use/toxicity
MH  - Indoles/*chemistry
MH  - Isoindoles/chemical synthesis/therapeutic use/toxicity
MH  - Mitochondria/metabolism
MH  - Neoplasms/drug therapy
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2010/10/12 06:00
MHDA- 2011/02/03 06:00
CRDT- 2010/10/09 06:00
PHST- 2010/10/09 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - JST.JSTAGE/cpb/58.1324 [pii]
AID - 10.1248/cpb.58.1324 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 2010 Oct;58(10):1324-7. doi: 10.1248/cpb.58.1324.