PMID- 20931533
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20141120
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 5
DP  - 2010 Oct
TI  - [Association of HER2 genetic heterogeneity with clinicopathological 
      characteristics in breast cancer].
PG  - 540-5
LID - 10.3760/cma.j.issn.1003-9406.2010.05.014 [doi]
AB  - OBJECTIVE: To introduce the College of American Pathologists/American College of 
      Medical Genetics Cytogenetics Resource Committee criteria for genetic 
      heterogeneity (GH) in HER2 testing, and investigate the clinicopathological 
      significance of HER2 genetic heterogeneity in invasive breast cancer. METHODS: 
      The clinical parameters of 100 cases of invasive breast carcinomas were 
      collected. HER2 expression level and HER2 gene copy number in formalin-fixed and 
      paraffin embedded tumor samples were detected by immunohistochemistry (IHC) and 
      fluorescence in situ hybridization (FISH), and the relationship between HER2 gene 
      GH and clinicopathological characteristics were analyzed. RESULTS: Among the 100 
      patients, HER2 gene GH was observed in 20 (20%) cases. When the number of HER2 
      amplified cells was more than 25%, the frequencies of FISH positive were higher 
      than those cases with less than 25% HER2 amplified cells. The results showed that 
      HER2 gene GH was associated with the degree of HER2 protein expression (P=0.004), 
      and ER expression (P=0.002). CONCLUSION: HER2 gene GH may be correlated with the 
      HER2 protein IHC 1+/2+, and ER expression in breast carcinoma. It is important 
      for doctors to avoid ignoring or only counting FISH positive cells leading to 
      incorrect diagnosis for these patients.
FAU - Yang, Yi-ling
AU  - Yang YL
AD  - Department of Breast Pathology, Tianjin Medical University Cancer Hospital, 
      Tianjin, P.R. China.
FAU - Fan, Yu
AU  - Fan Y
FAU - Lang, Rong-gang
AU  - Lang RG
FAU - Gu, Feng
AU  - Gu F
FAU - Fu, Li
AU  - Fu L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Receptors, Estrogen)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics/metabolism/*pathology
MH  - Carcinoma/*genetics/metabolism/*pathology
MH  - Female
MH  - Gene Dosage
MH  - Gene Expression Regulation, Neoplastic
MH  - *Genetic Heterogeneity
MH  - Humans
MH  - Middle Aged
MH  - Receptor, ErbB-2/*genetics/metabolism
MH  - Receptors, Estrogen/genetics/metabolism
EDAT- 2010/10/12 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/10/09 06:00
PHST- 2010/10/09 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - 940627108 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.05.014 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Oct;27(5):540-5. doi: 
      10.3760/cma.j.issn.1003-9406.2010.05.014.