PMID- 20931538
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20191210
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 5
DP  - 2010 Oct
TI  - [Application of fluorescence in situ hybridization technique in diagnosis of 
      variant Ph chromosome translocation and Ph-negative chronic myelocytic leukemia].
PG  - 563-6
LID - 10.3760/cma.j.issn.1003-9406.2010.05.019 [doi]
AB  - OBJECTIVE: To explore the value of fluorescence in situ hybridization (FISH) 
      technique in diagnosis of variant Ph chromosome translocation (VT) and Ph 
      chromosome-negative chronic myelocytic leukemia (CML). METHODS: Nine CML patients 
      with VT and 2 Ph chromosome-negative CML patients confirmed by R banding were 
      assayed with dual color-dual fusion BCR/ABL probe by FISH. RESULTS: The 9 
      patients with VT involved chromosomes 1, 3, 5, 12, 13, 15, 17 and 21 besides 
      chromosomes 9 and 22, and some of them showed recurrent aberrations; FISH results 
      were positive and the signal feature was 2R2G1Y. The 2 Ph-negative CML patients 
      had normal karyotypes; FISH was positive and the signal feature was 1R1G2Y and 
      1R1G1Y respectively. CONCLUSION: FISH can provide better diagnosis for CML with 
      VT and Ph-negative CML. Abnormal karyotype and marker gene changes can be 
      assessed based on the signal feature of the positive cell. So FISH is a 
      complementary method to banding technique in diagnosis of CML.
FAU - Jing, Yuan
AU  - Jing Y
AD  - Department of Hematology, the First Affiliated Hospital, Dalian Medical 
      University, Dalian, Liaoning, P.R. China. jingyuanan@yahoo.com.cn
FAU - Jiang, Feng
AU  - Jiang F
FAU - Lin, Shuang
AU  - Lin S
FAU - Wang, Fang-ting
AU  - Wang FT
FAU - Fang, Mei-yun
AU  - Fang MY
LA  - chi
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes/genetics
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Philadelphia Chromosome
MH  - *Translocation, Genetic
MH  - Young Adult
EDAT- 2010/10/12 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/10/09 06:00
PHST- 2010/10/09 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - 940627113 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.05.019 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Oct;27(5):563-6. doi: 
      10.3760/cma.j.issn.1003-9406.2010.05.019.