PMID- 20933279
OWN - NLM
STAT- MEDLINE
DCOM- 20110307
LR  - 20181201
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 32
IP  - 3
DP  - 2011 Jan
TI  - Bone regeneration using a microstereolithography-produced customized 
      poly(propylene fumarate)/diethyl fumarate photopolymer 3D scaffold incorporating 
      BMP-2 loaded PLGA microspheres.
PG  - 744-52
LID - 10.1016/j.biomaterials.2010.09.035 [doi]
AB  - Bony defects have been three-dimensionally (3D) created in many clinical 
      circumstances; however, many defects cannot be reconstructed because most of the 
      current bony substitutes cannot provide the necessary exact 3D structure. 
      Therefore, to overcome this limitation, a 3D scaffold with embedded growth 
      factor-delivering microspheres was developed by solid free-form fabrication (SFF) 
      technology using computer-aided design/manufacturing (CAD/CAM). In this study, 
      BMP-2-loaded poly(DL-lactic-co-glycolic acid) (PLGA) microspheres were 
      incorporated into a 3D scaffold that was fabricated using a 
      microstereolithography (MSTL) system with a suspension of microspheres and a 
      poly(propylene fumarate) (PPF)/diethyl fumarate (DEF) photopolymer. By measuring 
      release profiles in vitro, we verified that the fabricated microsphere-containing 
      3D scaffold could gradually release growth factor. The effects of BMP-2 were also 
      assessed in vitro by observing cell differentiation using MC3T3-E1 
      pre-osteoblasts. Finally, we confirmed that SFF scaffolds created by MSTL were 
      superior to traditional scaffolds produced using a particulate leaching/gas 
      foaming method. In addition, based on in vivo tests, the scaffolds that released 
      BMP-2 promoted bone formation. Based on these results, we concluded that our 3D 
      scaffold might be a useful tool for enhancing reconstruction quality in many 
      complex bony defects that should be reconstructed using a customized 3D scaffold.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Lee, Jin Woo
AU  - Lee JW
AD  - Department of Mechanical Engineering, The University of Texas, Austin, TX 
      78712-0292, USA.
FAU - Kang, Kyung Shin
AU  - Kang KS
FAU - Lee, Seung Ho
AU  - Lee SH
FAU - Kim, Jun-Young
AU  - Kim JY
FAU - Lee, Bu-Kyu
AU  - Lee BK
FAU - Cho, Dong-Woo
AU  - Cho DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101008
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Fumarates)
RN  - 0 (Polypropylenes)
RN  - 0 (poly(propylene fumarate))
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 5WBU5A3E8A (diethyl fumarate)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/*physiology
MH  - Cell Differentiation/physiology
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Fumarates/*chemistry
MH  - Lactic Acid/*chemistry
MH  - *Microspheres
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polypropylenes/*chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering/*methods
EDAT- 2010/10/12 06:00
MHDA- 2011/03/08 06:00
CRDT- 2010/10/12 06:00
PHST- 2010/07/24 00:00 [received]
PHST- 2010/09/14 00:00 [accepted]
PHST- 2010/10/12 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/03/08 06:00 [medline]
AID - S0142-9612(10)01190-7 [pii]
AID - 10.1016/j.biomaterials.2010.09.035 [doi]
PST - ppublish
SO  - Biomaterials. 2011 Jan;32(3):744-52. doi: 10.1016/j.biomaterials.2010.09.035. 
      Epub 2010 Oct 8.