PMID- 20933482
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20131121
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 878
IP  - 29
DP  - 2010 Nov 1
TI  - A plasma global metabolic profiling approach applied to an exercise study 
      monitoring the effects of glucose, galactose and fructose drinks during 
      post-exercise recovery.
PG  - 3015-23
LID - 10.1016/j.jchromb.2010.09.004 [doi]
AB  - A global metabolic profiling methodology based on gas chromatography coupled to 
      time-of-flight mass spectrometry (GC-TOFMS) for human plasma was applied to a 
      human exercise study focused on the effects of beverages containing glucose, 
      galactose, or fructose taken after exercise and throughout a recovery period of 6 
      h and 45 min. One group of 10 well trained male cyclists performed 3 experimental 
      sessions on separate days (randomized, single center). After performing a 
      standardized depletion protocol on a bicycle, subjects consumed one of three 
      different beverages: maltodextrin (MD)+glucose (2:1 ratio), MD+galactose (2:1), 
      and MD+fructose (2:1), consumed at an average of  approximately 1.25 g of carbohydrate (CHO) 
      ingested per minute. Blood was taken straight after exercise and every 45 min 
      within the recovery phase. With the resulting blood plasma, insulin, free fatty 
      acid (FFA) profile, glucose, and GC-TOFMS global metabolic profiling measurements 
      were performed. The resulting profiling data was able to match the results 
      obtained from the other clinical measurements with the addition of being able to 
      follow many different metabolites throughout the recovery period. The data 
      quality was assessed, with all the labelled internal standards yielding values of 
      <15% CV for all samples (n=335), apart from the labelled sucrose which gave a 
      value of 15.19%. Differences between recovery treatments including the appearance 
      of galactonic acid from the galactose based beverage were also highlighted.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Bruce, Stephen J
AU  - Bruce SJ
AD  - Nestle Research Center, Lausanne, Switzerland.
FAU - Breton, Isabelle
AU  - Breton I
FAU - Decombaz, Jacques
AU  - Decombaz J
FAU - Boesch, Chris
AU  - Boesch C
FAU - Scheurer, Eva
AU  - Scheurer E
FAU - Montoliu, Ivan
AU  - Montoliu I
FAU - Rezzi, Serge
AU  - Rezzi S
FAU - Kochhar, Sunil
AU  - Kochhar S
FAU - Guy, Philippe A
AU  - Guy PA
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20100921
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Blood Glucose)
RN  - 0 (Fatty Acids)
RN  - 0 (Insulin)
RN  - 30237-26-4 (Fructose)
RN  - IY9XDZ35W2 (Glucose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Adult
MH  - Beverages/*analysis
MH  - Blood Glucose/analysis
MH  - *Exercise
MH  - Fatty Acids/blood/metabolism
MH  - Fructose/blood/*metabolism
MH  - Galactose/blood/*metabolism
MH  - Glucose/*metabolism
MH  - Humans
MH  - Insulin/blood/metabolism
MH  - Male
MH  - Metabolome
MH  - Young Adult
EDAT- 2010/10/12 06:00
MHDA- 2011/01/14 06:00
CRDT- 2010/10/12 06:00
PHST- 2010/02/19 00:00 [received]
PHST- 2010/09/01 00:00 [revised]
PHST- 2010/09/02 00:00 [accepted]
PHST- 2010/10/12 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
AID - S1570-0232(10)00580-5 [pii]
AID - 10.1016/j.jchromb.2010.09.004 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Nov 1;878(29):3015-23. doi: 
      10.1016/j.jchromb.2010.09.004. Epub 2010 Sep 21.