PMID- 20936260
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20240322
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 398
IP  - 7-8
DP  - 2010 Dec
TI  - Rapid direct analysis in real time (DART) mass spectrometric detection of 
      juvenile hormone III and its terpene precursors.
PG  - 3005-13
LID - 10.1007/s00216-010-4269-4 [doi]
AB  - Direct analysis in real time (DART) is a plasma-based ambient ionization 
      technique that enables rapid ionization of small molecules with high sample 
      throughput. In this work, DART was coupled to an orthogonal (oa) time-of-flight 
      (TOF) mass spectrometer and the system was optimized for analyzing a vital 
      hormonal regulator in insects, juvenile hormone (JH) III and its terpene 
      precursors, namely, farnesol, farnesoic acid, and methyl farnesoate. Optimization 
      experiments were planned using design of experiments (DOE) full factorial models 
      to identify the most significant DART variables contributing to JH III analysis 
      sensitivity by DART-TOF mass spectrometry (MS). The optimized DART-TOF MS method 
      had femtomole to sub-picomole detection limits for terpene standards, along with 
      mass accuracies below 5 ppm. Finally, the possibility of distinguishing between 
      two farnesol isomers by in-source-collision-induced dissociation (CID) in the 
      first differentially pumped region of the oaTOF mass spectrometer was 
      investigated. DART-MS enabled high-throughput, sensitive analysis with 
      acquisition times ranging from 30 s to a minute. To the best of our knowledge, 
      this is the first report on the application of DART-MS to the detection and 
      identification of volatile or semi-volatile insect terpenoids, and on the use of 
      DOE approaches to optimize DART-MS analytical procedures.
FAU - Navare, Arti T
AU  - Navare AT
AD  - School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 
      Atlantic Dr. NW, Atlanta, GA 30332-0400, USA.
FAU - Mayoral, Jaime G
AU  - Mayoral JG
FAU - Nouzova, Marcela
AU  - Nouzova M
FAU - Noriega, Fernando G
AU  - Noriega FG
FAU - Fernandez, Facundo M
AU  - Fernandez FM
LA  - eng
GR  - R01 AI045545/AI/NIAID NIH HHS/United States
GR  - R01 AI045545-06/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20101009
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Sesquiterpenes)
RN  - 0 (Terpenes)
RN  - B74U6BJ6J5 (juvenile hormone III)
SB  - IM
MH  - Animals
MH  - Mass Spectrometry/*methods
MH  - Random Allocation
MH  - Sesquiterpenes/*analysis
MH  - Terpenes/*analysis
PMC - PMC3040246
MID - NIHMS255242
EDAT- 2010/10/12 06:00
MHDA- 2011/04/08 06:00
PMCR- 2011/12/01
CRDT- 2010/10/12 06:00
PHST- 2010/08/26 00:00 [received]
PHST- 2010/09/28 00:00 [accepted]
PHST- 2010/10/12 06:00 [entrez]
PHST- 2010/10/12 06:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - 10.1007/s00216-010-4269-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Dec;398(7-8):3005-13. doi: 10.1007/s00216-010-4269-4. 
      Epub 2010 Oct 9.