PMID- 20937133
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20211020
IS  - 1742-2094 (Electronic)
IS  - 1742-2094 (Linking)
VI  - 7
DP  - 2010 Oct 11
TI  - Cerebrovascular expression of proteins related to inflammation, oxidative stress 
      and neurotoxicity is altered with aging.
PG  - 63
LID - 10.1186/1742-2094-7-63 [doi]
AB  - BACKGROUND: Most neurodegenerative diseases are age-related disorders; however, 
      how aging predisposes the brain to disease has not been adequately addressed. The 
      objective of this study is to determine whether expression of proteins in the 
      cerebromicrovasculature related to inflammation, oxidative stress and 
      neurotoxicity is altered with aging. METHODS: Brain microvessels are isolated 
      from Fischer 344 rats at 6, 12, 18 and 24 months of age. Levels of interleukin 
      (IL)-1beta and IL-6 RNA are determined by RT-PCR and release of cytokines into the 
      media by ELISA. Vessel conditioned media are also screened by ELISA for IL-1alpha, 
      monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha, (TNFalpha), and 
      interferon gamma (IFNgamma). Immunofluorescent analysis of brain sections for IL-1beta and 
      IL-6 is performed. RESULTS: Expression of IL-1beta and IL-6, both at RNA and protein 
      levels, significantly (p < 0.01) decreases with age. Levels of MCP-1, TNFalpha, 
      IL-1alpha, and IFNgamma are significantly (p < 0.05-0.01) lower in 24 month old rats 
      compared to 6 month old animals. Immunofluorescent analysis of brain vessels also 
      shows a decline in IL-1beta and IL-6 in aged rats. An increase in oxidative stress, 
      assessed by increased carbonyl formation, as well as a decrease in the 
      antioxidant protein manganese superoxide dismutase (MnSOD) is evident in vessels 
      of aged animals. Finally, addition of microvessel conditioned media from aged 
      rats to neuronal cultures evokes significant (p < 0.001) neurotoxicity. 
      CONCLUSIONS: These data demonstrate that cerebrovascular expression of proteins 
      related to inflammation, oxidative stress and neurotoxicity is altered with aging 
      and suggest that the microvasculature may contribute to functional changes in the 
      aging brain.
FAU - Tripathy, Debjani
AU  - Tripathy D
AD  - Garrison Institute on Aging, Texas Tech University Health Sciences Center, 
      Lubbock, Texas, USA.
FAU - Yin, Xiangling
AU  - Yin X
FAU - Sanchez, Alma
AU  - Sanchez A
FAU - Luo, Jinhua
AU  - Luo J
FAU - Martinez, Joseph
AU  - Martinez J
FAU - Grammas, Paula
AU  - Grammas P
LA  - eng
GR  - AG020569/AG/NIA NIH HHS/United States
GR  - AG028367/AG/NIA NIH HHS/United States
GR  - AG15964/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20101011
PL  - England
TA  - J Neuroinflammation
JT  - Journal of neuroinflammation
JID - 101222974
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Aging/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Cell Death
MH  - Cells, Cultured
MH  - Cerebral Cortex/*metabolism
MH  - Chemokine CCL2/genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Inflammation
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-1beta/genetics/*metabolism
MH  - Interleukin-6/genetics/*metabolism
MH  - Male
MH  - Microvessels/*metabolism
MH  - Neurons/cytology/metabolism
MH  - Oxidative Stress/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
PMC - PMC2965134
EDAT- 2010/10/13 06:00
MHDA- 2010/12/14 06:00
PMCR- 2010/10/11
CRDT- 2010/10/13 06:00
PHST- 2010/07/01 00:00 [received]
PHST- 2010/10/11 00:00 [accepted]
PHST- 2010/10/13 06:00 [entrez]
PHST- 2010/10/13 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2010/10/11 00:00 [pmc-release]
AID - 1742-2094-7-63 [pii]
AID - 10.1186/1742-2094-7-63 [doi]
PST - epublish
SO  - J Neuroinflammation. 2010 Oct 11;7:63. doi: 10.1186/1742-2094-7-63.