PMID- 20939004
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20181217
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 108
IP  - 2
DP  - 2011 Feb
TI  - Pancreatic cell immobilization in alginate beads produced by emulsion and 
      internal gelation.
PG  - 424-34
LID - 10.1002/bit.22959 [doi]
AB  - Alginate has been used to protect transplanted pancreatic islets from immune 
      rejection and as a matrix to increase the insulin content of islet progenitor 
      cells. The throughput of alginate bead generation by the standard extrusion and 
      external gelation method is limited by the rate of droplet formation from 
      nozzles. Alginate bead generation by emulsion and internal gelation is a 
      scaleable alternative that has been used with biological molecules and microbial 
      cells, but not mammalian cells. We describe the novel adaptation of this process 
      to mammalian cell immobilization. After optimization, the emulsion process 
      yielded 90 +/- 2% mouse insulinoma 6 (MIN6) cell survival, similar to the extrusion 
      process. The MIN6 cells expanded at the same rate in both bead types to form 
      pseudo-islets with increased glucose stimulation index compared to cells in 
      suspension. The emulsion process was suitable for primary pancreatic exocrine 
      cell immobilization, leading to 67 +/- 32 fold increased insulin expression after 
      10 days of immobilized culture. Due to the scaleability and broad availability of 
      stirred mixers, the emulsion process represents an attractive option for 
      laboratories that are not equipped with extrusion-based cell encapsulators, as 
      well as for the production of immobilized or encapsulated cellular therapeutics 
      on a clinical scale.
CI  - (c) 2010 Wiley Periodicals, Inc.
FAU - Hoesli, Corinne A
AU  - Hoesli CA
AD  - Department of Chemical and Biological Engineering, University of British 
      Columbia, Vancouver, BC, Canada.
FAU - Raghuram, Kamini
AU  - Raghuram K
FAU - Kiang, Roger L J
AU  - Kiang RL
FAU - Mocinecova, Dusana
AU  - Mocinecova D
FAU - Hu, Xiaoke
AU  - Hu X
FAU - Johnson, James D
AU  - Johnson JD
FAU - Lacik, Igor
AU  - Lacik I
FAU - Kieffer, Timothy J
AU  - Kieffer TJ
FAU - Piret, James M
AU  - Piret JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Alginates)
RN  - 0 (Emulsions)
RN  - 0 (Hexuronic Acids)
RN  - 0 (Insulin)
RN  - 8A5D83Q4RW (Glucuronic Acid)
SB  - IM
MH  - *Alginates
MH  - Animals
MH  - Biotechnology/*methods
MH  - Cell Line
MH  - Cell Proliferation
MH  - *Cells, Immobilized
MH  - Emulsions
MH  - Glucuronic Acid
MH  - Hexuronic Acids
MH  - Insulin/metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*physiology
MH  - Mice
MH  - *Microspheres
EDAT- 2010/10/13 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/10/13 06:00
PHST- 2010/10/13 06:00 [entrez]
PHST- 2010/10/13 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - 10.1002/bit.22959 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2011 Feb;108(2):424-34. doi: 10.1002/bit.22959.