PMID- 20939045
OWN - NLM
STAT- MEDLINE
DCOM- 20110301
LR  - 20131121
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 77
IP  - 11
DP  - 2010 Nov
TI  - Identification of estrogen-responsive vitelline envelope protein fragments from 
      rainbow trout (Oncorhynchus mykiss) plasma using mass spectrometry.
PG  - 963-70
LID - 10.1002/mrd.21244 [doi]
AB  - Plasma peptides previously associated with exposure of juvenile male rainbow 
      trout (Oncorhynchus mykiss) to the hormone 17beta-estradiol (E2) were identified 
      using matrix-assisted laser desorption ionization time of flight mass 
      spectrometry (MALDI-TOF-MS). Specifically, plasma peptides of interest were 
      fractionated and subsequently identified via spectra obtained by MALDI QqTOF 
      MS/MS and LC-MALDI TOFTOF MS/MS analysis, de novo sequencing and database 
      matching. The two peptide masses were identified as significant matches for 
      fragments of the C-terminal propeptides from rainbow trout vitelline envelope 
      protein (VEP)alpha and VEPgamma isoforms. Our findings document the presence of the 
      C-terminal propeptides from rainbow trout VEPalpha and VEPgamma proteins in the 
      bloodstream of juvenile male rainbow trout exposed to E2 via MALDI-TOF-MS 
      detection. We provide three possible explanations for the presence of C-terminal 
      propeptides in the bloodstream, as well as compare previously obtained hepatic 
      transcriptomic results with the plasma proteomic results obtained in the present 
      study.
CI  - Published 2010 Wiley-Liss, Inc.
FAU - Salinas, K
AU  - Salinas K
AD  - U.S. Environmental Protection Agency, National Health and Environmental Effects 
      Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, 
      FL 32514, USA. salinas.kimberly@epa.gov
FAU - Hemmer, M J
AU  - Hemmer MJ
FAU - Serrano, J
AU  - Serrano J
FAU - Higgins, L
AU  - Higgins L
FAU - Anderson, L B
AU  - Anderson LB
FAU - Benninghoff, A D
AU  - Benninghoff AD
FAU - Williams, D E
AU  - Williams DE
FAU - Walker, C
AU  - Walker C
LA  - eng
GR  - ES00210/ES/NIEHS NIH HHS/United States
GR  - ES003850/ES/NIEHS NIH HHS/United States
GR  - ES013534/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101011
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Biomarkers, Pharmacological)
RN  - 0 (Egg Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (vitelline membrane proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biomarkers, Pharmacological/*analysis/blood/chemistry/metabolism
MH  - Blood Chemical Analysis/methods
MH  - Egg Proteins/*analysis/blood/chemistry/metabolism
MH  - Estradiol/*pharmacology
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Oncorhynchus mykiss/*blood/metabolism
MH  - Peptide Fragments/analysis/blood/metabolism
MH  - Sequence Analysis, Protein/methods
MH  - Vitelline Membrane/chemistry/drug effects/metabolism
EDAT- 2010/10/13 06:00
MHDA- 2011/03/02 06:00
CRDT- 2010/10/13 06:00
PHST- 2010/04/09 00:00 [received]
PHST- 2010/08/28 00:00 [accepted]
PHST- 2010/10/13 06:00 [entrez]
PHST- 2010/10/13 06:00 [pubmed]
PHST- 2011/03/02 06:00 [medline]
AID - 10.1002/mrd.21244 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2010 Nov;77(11):963-70. doi: 10.1002/mrd.21244. Epub 2010 Oct 11.