PMID- 20939484
OWN - NLM
STAT- MEDLINE
DCOM- 20110111
LR  - 20161125
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 24
IP  - 9
DP  - 2010 Sep
TI  - [Effect of simvastatin on inducing endothelial progenitor cells homing and 
      promoting bone defect repair].
PG  - 1103-6
AB  - OBJECTIVE: To investigate the effect of simvastatin on inducing endothelial 
      progenitor cells (EPCs) homing and promoting bone defect repair, and to explore 
      the mechanism of local implanting simvastatin in promoting bone formation. 
      METHODS: Simvastatin (50 mg) compounded with polylactic acid (PLA, 200 mg) or 
      only PLA (200 mg) was dissolved in acetone (1 mL) to prepare implanted materials 
      (Simvastatin-PLA material, PLA material). EPCs were harvested from bone marrow of 
      2 male rabbits and cultured with M199; after identified by immunohistochemistry, 
      the cell suspension of EPCs at the 3rd generation (2 x 10(6) cells/mL) was 
      prepared and transplanted into 12 female rabbits through auricular veins (2 mL). 
      After 3 days, the models of cranial defect with 15 cm diameter were made in the 
      12 female rabbits. And the defects were repaired with Simvastatin-PLA materials 
      (experimental group, n=6) and PLA materials (control group, n=6), respectively. 
      The bone repair was observed after 8 weeks of operation by gross appearance, 
      X-ray film, and histology; gelatin-ink perfusion and HE staining were used to 
      show the new vessels formation in the defect. Fluorescence in situ hybridization 
      (FISH) was performed to show the EPCs homing at the defect site. RESULTS: All 
      experimental animals of 2 groups survived to the end of the experiment. After 8 
      weeks in experimental group, new bone formation was observed in the bone defect 
      by gross and histology, and an irregular, hyperdense shadow by X-ray film; no 
      similar changes were observed in control group. FISH showed that the male EPC 
      containing Y chromosome was found in the wall of new vessels in the defect of 
      experimental group, while no male EPC containing Y chromosome was found in 
      control group. The percentage of new bone formation in defect area was 91.63% +/- 
      4.07% in experimental group and 59.45% +/- 5.43% in control group, showing 
      significant difference (P < 0.05). CONCLUSION: Simvastatin can promote bone 
      defect repair, and its mechanism is probably associated with inducing EPCs homing 
      and enhancing vasculogenesis.
FAU - Song, Quansheng
AU  - Song Q
AD  - Department of Orthopaedics, Third Hospital, Peking University, Beijing, 100191, 
      PR China.
FAU - Wang, Lingying
AU  - Wang L
FAU - Zhu, Jinglin
AU  - Zhu J
FAU - Han, Xiaoguang
AU  - Han X
FAU - Li, Xu
AU  - Li X
FAU - Yang, Yanlin
AU  - Yang Y
FAU - Sun, Yan
AU  - Sun Y
FAU - Song, Chunli
AU  - Song C
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - AGG2FN16EV (Simvastatin)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/*drug effects
MH  - Cells, Cultured
MH  - Endothelial Cells/cytology/*drug effects
MH  - Female
MH  - Lactic Acid/pharmacology
MH  - Male
MH  - Polyesters
MH  - Polymers/pharmacology
MH  - Rabbits
MH  - Simvastatin/*pharmacology
MH  - Stem Cells/cytology/*drug effects
MH  - Tissue Engineering
EDAT- 2010/10/14 06:00
MHDA- 2011/01/12 06:00
CRDT- 2010/10/14 06:00
PHST- 2010/10/14 06:00 [entrez]
PHST- 2010/10/14 06:00 [pubmed]
PHST- 2011/01/12 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Sep;24(9):1103-6.