PMID- 20940520
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20190513
IS  - 1349-9157 (Electronic)
IS  - 0449-3060 (Linking)
VI  - 51
IP  - 6
DP  - 2010
TI  - Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature 
      hippocampal neurons.
PG  - 627-31
AB  - The direct biological effects of radiation, particularly accelerated heavy 
      particle ions, on neurons are not fully known. Hence, the direct effect of 
      carbon-ion beams on immature neurons was investigated by comparing to the effect 
      of X-rays in vitro using primary hippocampal neurons. Primary neurons were 
      prepared from hippocampi of fetal rats at embryonic day 18 from timed pregnant 
      Wistar rats and cultured with Banker's methods. At 7 Days In Vitro (DIV), the 
      cells were irradiated with 140 kV X-ray and 18.3 MeV/amu carbon-ion beams (LET = 
      108 keV/microm). The cells were fixed with 4% paraformaldehyde at 12 hours after 
      irradiation. Then, the cells were treated with terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and DAPI staining for 
      measuring the percentage of apoptosis (apoptotic index: AI). AI in 
      sham-irradiated hippocampal neurons was 18%. The value of AI (AIs) of the cells 
      irradiated with X-rays at 10 or 30 Gy were 15% or 23%, respectively. AI in cells 
      irradiated with carbon-ion beams at 1 Gy, 3 Gy, 5 Gy and 10 Gy were 22%, 23%, 24% 
      and 33%, respectively. AI was significantly increased by carbon-ion beams at 10 
      Gy (p < 0.001). The apoptosis of hippocampal neurons increased in a 
      dose-dependent manner following both X-ray and carbon-ion beams irradiation. 
      Carbon-ion beams were about 10-fold more effective than X-rays for apoptosis 
      induction in immature hippocampal neurons.
FAU - Kaminuma, Takuya
AU  - Kaminuma T
AD  - Department of Radiation Oncology, Gunma University Graduate School of Medicine, 
      Maebashi, Gunma, Japan.
FAU - Suzuki, Yoshiyuki
AU  - Suzuki Y
FAU - Shirai, Katsuyuki
AU  - Shirai K
FAU - Mizui, Toshiyuki
AU  - Mizui T
FAU - Noda, Shin-ei
AU  - Noda SE
FAU - Yoshida, Yukari
AU  - Yoshida Y
FAU - Funayama, Tomoo
AU  - Funayama T
FAU - Takahashi, Takeo
AU  - Takahashi T
FAU - Kobayashi, Yasuhiko
AU  - Kobayashi Y
FAU - Shirao, Tomoaki
AU  - Shirao T
FAU - Nakano, Takashi
AU  - Nakano T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101006
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Animals
MH  - Apoptosis/*radiation effects
MH  - Carbon
MH  - Female
MH  - Heavy Ion Radiotherapy
MH  - Heavy Ions/*adverse effects
MH  - Hippocampus/cytology/embryology/*radiation effects
MH  - In Vitro Techniques
MH  - Neurons/cytology/radiation effects
MH  - Pregnancy
MH  - Radiation Tolerance
MH  - Rats
MH  - Rats, Wistar
MH  - Relative Biological Effectiveness
MH  - X-Rays
EDAT- 2010/10/14 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/10/14 06:00
PHST- 2010/10/14 06:00 [entrez]
PHST- 2010/10/14 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - JST.JSTAGE/jrr/10050 [pii]
AID - 10.1269/jrr.10050 [doi]
PST - ppublish
SO  - J Radiat Res. 2010;51(6):627-31. doi: 10.1269/jrr.10050. Epub 2010 Oct 6.