PMID- 20941606
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20171116
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 677
DP  - 2011
TI  - DCs in immune tolerance in steady-state conditions.
PG  - 113-26
LID - 10.1007/978-1-60761-869-0_8 [doi]
AB  - Dendritic cells (DCs) are antigen-presenting cells (APCs) characterized by a 
      unique capacity to stimulate naive T cells and initiate primary immune responses. 
      Recent studies suggest that DCs are also involved in the induction of 
      immunological tolerance in peripheral tissues under steady-state conditions by 
      maintaining the homeostasis of self-reactive CD4(+)Foxp3(+)naturally occurring 
      thymic-derived regulatory T cells (nT(regs)) and de novo generation of 
      antigen-specific CD4(+)Foxp3(+)inducible regulatory T cells (iT(regs)). We 
      demonstrate here the impact of CD11(+)DCs on the antigen-specific differentiation 
      of CD4(+)Foxp3(+)iT(regs) from CD4(+)Foxp3(-)T cells under steady-state and 
      inflammatory conditions. CD11c(+)DCs promoted the transforming growth factor 
      (TGF)-beta1-mediated conversion of CD4(+)Foxp3(-)T cells into CD4(+)Foxp3(+)iT(regs) 
      in vitro, while stimulation of CD11c(+)DCs with CpG oligodeoxynucleotide (ODN) 
      abrogated this conversion. Furthermore, antigen-specific generation of 
      CD4(+)Foxp3(+)iT(regs) required the function of CD11(+)DCs under steady-state 
      conditions, whereas such conversion was severely abolished under inflammatory 
      conditions. Thus, these results suggest the crucial role of DCs in the 
      antigen-specific de novo conversion of CD4(+)Foxp3(-)T cells into 
      CD4(+)Foxp3(+)iT(regs) under steady-state conditions, thereby leading to the 
      establishment of peripheral immune tolerance.
FAU - Fukaya, Tomohiro
AU  - Fukaya T
AD  - Laboratory for Dendritic Cell Immunobiology, RIKEN Research Center for Allergy 
      and Immunology, Kanagawa, Japan.
FAU - Takagi, Hideaki
AU  - Takagi H
FAU - Taya, Honami
AU  - Taya H
FAU - Sato, Katsuaki
AU  - Sato K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (CD4 Antigens)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/*immunology
MH  - CD4 Antigens/immunology
MH  - Cell Differentiation/*physiology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Immune Tolerance/immunology/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes/immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Thymus Gland/cytology
EDAT- 2010/10/14 06:00
MHDA- 2011/02/03 06:00
CRDT- 2010/10/14 06:00
PHST- 2010/10/14 06:00 [entrez]
PHST- 2010/10/14 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-869-0_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;677:113-26. doi: 10.1007/978-1-60761-869-0_8.