PMID- 20941608
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20181201
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 677
DP  - 2011
TI  - In vitro-generated DC with tolerogenic functions: perspectives for in vivo 
      cellular therapy.
PG  - 149-59
LID - 10.1007/978-1-60761-869-0_10 [doi]
AB  - Dendritic cells (DCs) have a central role in immune regulation and serve as an 
      essential link between innate and adaptive immunity. Their broad range of 
      powerful immune stimulatory as well as regulatory functions has made DCs a target 
      for vaccine development strategies. One approach to promote the tolerogenicity of 
      DCs is to suppress their maturation by pharmacological agents, including the 
      glucocorticoid dexamethasone. In this chapter, we describe methods to generate 
      tolerogenic Dex-DC derived from either human peripheral blood monocytes or rat 
      bone marrow cells.
FAU - van Kooten, Cees
AU  - van Kooten C
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands. kooten@lumc.nl
FAU - Gelderman, Kyra A
AU  - Gelderman KA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cytokines)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Organic Chemicals)
RN  - 0 (Tolerogen)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/immunology
MH  - Cell Differentiation/physiology
MH  - Cell- and Tissue-Based Therapy/*statistics & numerical data
MH  - Cells, Cultured
MH  - Cytokines/pharmacology
MH  - Dendritic Cells/*drug effects/immunology
MH  - Dexamethasone/pharmacology
MH  - Humans
MH  - Immune Tolerance/*drug effects
MH  - Immunosuppressive Agents/pharmacology
MH  - Internationality
MH  - Organic Chemicals/toxicity
MH  - Rats
EDAT- 2010/10/14 06:00
MHDA- 2011/02/03 06:00
CRDT- 2010/10/14 06:00
PHST- 2010/10/14 06:00 [entrez]
PHST- 2010/10/14 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-869-0_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;677:149-59. doi: 10.1007/978-1-60761-869-0_10.