PMID- 20941613
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20211203
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 677
DP  - 2011
TI  - Antiinflammatory and immunosuppressive functions of mast cells.
PG  - 207-20
LID - 10.1007/978-1-60761-869-0_15 [doi]
AB  - Through the release of biologically active products, mast cells function as 
      important effector and immunoregulatory cells in diverse immunological reactions 
      and other biological responses; for example, mast cells promote inflammation and 
      other tissue changes in immunoglobulin E (IgE)-associated allergic disorders, as 
      well as in certain innate and adaptive immune responses that are thought to be 
      independent of IgE. Despite the mast cell's well-deserved reputation as a 
      promoter of inflammation, others and we have used bone marrow-derived cultured 
      mast cell (BMCMC) engrafted mast cell-deficient c-kit-mutant mice (so-called 
      "mast cell knock-in" mice) to show that mast cells can also have important 
      antiinflammatory and immunosuppressive functions in vivo. An early study showed 
      that mast cells can contribute to susceptibility to ultraviolet B (UVB)-induced 
      immunosuppression in one model of contact hypersensitivity (CHS), through effects 
      mediated at least in part by histamine. Subsequently, it was reported that mast 
      cells can mediate negative immunomodulatory effects following Anopheles mosquito 
      bites, and in peripheral tolerance to skin allografts; however, the mechanism(s) 
      by which mast cells mediate immunosuppressive functions in these two studies 
      remains to be elucidated. Finally, we showed that mast cells and mast 
      cell-derived IL-10 can limit the magnitude of and promote the resolution of 
      certain CHS responses, and suppress the inflammation and skin injury associated 
      with innate cutaneous responses to chronic low-dose UVB irradiation. This chapter 
      outlines the generation of BMCMCs, a powerful model system commonly used to: (1) 
      identify potential mast cell mediators in vitro; (2) study the mechanisms of mast 
      cell activation and mediator release in response to specific stimuli in vitro; 
      and (3) engraft mast cell-deficient mice to study the effector and 
      immunoregulatory roles of mast cells or specific mast cell mediators in diverse 
      immunological responses in vivo.
FAU - Kalesnikoff, Janet
AU  - Kalesnikoff J
AD  - Department of Pathology, Stanford University School of Medicine, Stanford, CA, 
      USA. jkalesni@stanford.edu
FAU - Galli, Stephen J
AU  - Galli SJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Immunosuppressive Agents)
RN  - 130068-27-8 (Interleukin-10)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - Bone Marrow Cells/drug effects/immunology
MH  - Cells, Cultured
MH  - Histamine/pharmacology
MH  - Immunoglobulin E/*adverse effects/immunology
MH  - Immunosuppression Therapy
MH  - Immunosuppressive Agents/adverse effects/*immunology
MH  - Inflammation/drug therapy/immunology
MH  - Interleukin-10/immunology/*therapeutic use
MH  - Mast Cells/drug effects/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
EDAT- 2010/10/14 06:00
MHDA- 2011/02/03 06:00
CRDT- 2010/10/14 06:00
PHST- 2010/10/14 06:00 [entrez]
PHST- 2010/10/14 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-869-0_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2011;677:207-20. doi: 10.1007/978-1-60761-869-0_15.